Mohapatra Bidyut R, Broersma Klaas, Nordin Rick, Mazumder Asit
Water and Watershed Research Program, Department of Biology, University of Victoria, 3800 Finnerty Road, Victoria, British Columbia, Canada.
Microbiol Immunol. 2007;51(8):733-40. doi: 10.1111/j.1348-0421.2007.tb03962.x.
The objective of this study was to investigate the potential of repetitive extragenic palindromic anchored polymerase chain reaction (rep-PCR) in differentiating fecal Escherichia coli isolates of human, domestic- and wild-animal origin that might be used as a molecular tool to identify the possible source(s) of fecal pollution of source water. A total of 625 fecal E. coli isolates of human, 3 domestic- (cow, dog and horse) and 7 wild-animal (black bear, coyote, elk, marmot, mule deer, raccoon and wolf) species were characterized by rep-PCR DNA fingerprinting technique coupled with BOX A1R primer and discriminant analysis. Discriminant analysis of rep-PCR DNA fingerprints of fecal E. coli isolates from 11 host sources revealed an average rate of correct classification of 79.89%, and 84.6%, 83.8%, 83.3%, 82.5%, 81.6%, 80.8%, 79.8%, 79.3%, 77.4%, 73.2% and 63.6% of elk, human, marmot, mule deer, cow, coyote, raccoon, horse, dog, wolf and black bear fecal E. coli isolates were assigned to the correct host source. These results suggest that rep-PCR DNA fingerprinting procedures can be used as a source tracking tool for detection of human- as well as animal-derived fecal contamination of water.
本研究的目的是调查重复基因外回文锚定聚合酶链反应（rep-PCR）在区分人、家畜和野生动物源粪便大肠杆菌分离株方面的潜力，该技术可作为一种分子工具来识别水源粪便污染的可能来源。采用rep-PCR DNA指纹技术结合BOX A1R引物和判别分析，对总共625株人源、3种家畜（牛、狗和马）源以及7种野生动物（黑熊、郊狼、麋鹿、土拨鼠、骡鹿、浣熊和狼）源的粪便大肠杆菌分离株进行了特征分析。对来自11种宿主来源的粪便大肠杆菌分离株的rep-PCR DNA指纹进行判别分析，结果显示平均正确分类率为79.89%，麋鹿、人、土拨鼠、骡鹿、牛、郊狼、浣熊、马、狗、狼和黑熊粪便大肠杆菌分离株分别有84.6%、83.8%、83.3%、82.5%、81.
