人真核生物延伸因子1Bγ的磷酸化受紫杉醇调控。

Phosphorylation of human eukaryotic elongation factor 1Bgamma is regulated by paclitaxel.

作者信息

Prado Miguel A, Casado Pedro, Zuazua-Villar Pedro, del Valle Eva, Artime Noelia, Cabal-Hierro Lucía, Martínez-Campa Carlos M, Lazo Pedro S, Ramos Sofía

机构信息

Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, Oviedo, Spain.

出版信息

Proteomics. 2007 Sep;7(18):3299-304. doi: 10.1002/pmic.200700226.

DOI:10.1002/pmic.200700226

PMID:17708594

Abstract

Paclitaxel (Ptx) is an antitumoural drug that inhibits microtubule dynamics, causes G2/M arrest and induces cell death. 2-D PAGE and MALDI-TOF-MS analysis of HeLa cells extracts revealed that Ptx up-regulates a form of the eukaryotic elongation factor 1Bgamma (eEF1Bgamma) and down-regulates another one. This event, linked to the lack of Ptx effect over eEF1Bgamma mRNA or protein levels suggested a PTM of this elongation factor. Further 2-D PAGE analysis followed by a phosphospecific staining with PRO-Q Diamond showed the staining of the Ptx up-regulated form only. Moreover, this Ptx up-regulated form of eEF1Bgamma disappears upon treatment with protein phosphatase. Thus, we demonstrate that human eEF1Bgamma phosphorylation is regulated by Ptx.

摘要

紫杉醇（Ptx）是一种抗肿瘤药物，它能抑制微管动力学，导致G2/M期阻滞并诱导细胞死亡。对HeLa细胞提取物进行的二维聚丙烯酰胺凝胶电泳（2-D PAGE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析表明，Ptx上调了真核延伸因子1Bγ（eEF1Bγ）的一种形式，并下调了另一种形式。这一事件与Ptx对eEF1Bγ mRNA或蛋白质水平缺乏影响有关，提示该延伸因子存在翻译后修饰（PTM）。进一步的二维聚丙烯酰胺凝胶电泳分析，随后用PRO-Q Diamond进行磷酸特异性染色，结果显示仅对Ptx上调的形式进行了染色。此外，用蛋白磷酸酶处理后，这种Ptx上调的eEF1Bγ形式消失。因此，我们证明了人eEF1Bγ的磷酸化受Ptx调控。