[人乙肝病毒核心蛋白和土拨鼠乙肝病毒核心蛋白中的常见线性B细胞表位]

[Common linear B-cell epitopes in human hepatitis B virus core protein and woodchuck hepatitis virus core protein].

作者信息

Zhang Zhen-hua, Wang Rong, Tian Yong-jun, Li Lei, Xia Jian-bo, Gong Jin-song, Lu Meng-ji, Gong Fei-li, Yang Dong-liang

机构信息

Division of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2007 Aug;15(8):605-8.

PMID:17711632

Abstract

OBJECTIVE

To search for and verify some common B cell epitopes in the core proteins of woodchuck hepatitis virus and human hepatitis B virus.

METHODS

Monoclonal antibodies against both core proteins of woodchuck hepatitis virus (WHV) and human hepatitis B virus (HBV) were prepared by inoculating Balb/c mice with denatured recombination WHV and HBV core proteins. ELISA and immunoblotting assays for WHcAg and HBcAg were carried out by using these antibodies. Immunohistochemistry was carried out with liver tissue sections of both WHV-infected woodchucks and chronic HBV-infected patients. The epitopes were mapped with the mouse mAbs (6D1 and 1H4) by using a panel of 24 16mer overlapping peptides covering the entire WHcAg. The amino acid sequences of WHcAg and HBcAg were compared.

RESULTS

Cross-reactions were observed between mAbs (6D1 and 1H4) and WHcAg and between Mabs and HBcAg/HBcAg in ELISA and immunoblotting assay. Liver tissue sections of both WHV-infected woodchucks and chronic HBV-infected patients could be stained specifically by mAbs. The epitopes were mapped at aa1-8 (6D1) and aa125-140 (1H4) of the core proteins of both WHV and HBV by using ELISA assay. WHcAg and HBcAg share similar amino acids sequences at aa1-8 and aa125-140 respectively.

CONCLUSION

The core proteins of woodchuck hepatitis virus and human hepatitis B virus share common linear B cell epitopes which span aa1-8 and aa125-140 respectively.

摘要

目的

寻找并验证土拨鼠肝炎病毒和人类乙型肝炎病毒核心蛋白中的一些常见B细胞表位。

方法

通过用变性重组土拨鼠肝炎病毒（WHV）和人类乙型肝炎病毒（HBV）核心蛋白接种Balb/c小鼠，制备针对WHV和HBV核心蛋白的单克隆抗体。使用这些抗体对WHcAg和HBcAg进行ELISA和免疫印迹分析。对感染WHV的土拨鼠和慢性HBV感染患者的肝组织切片进行免疫组织化学分析。通过使用一组覆盖整个WHcAg的24个16聚体重叠肽，用小鼠单克隆抗体（6D1和1H4）对表位进行定位。比较WHcAg和HBcAg的氨基酸序列。

结果

在ELISA和免疫印迹分析中，观察到单克隆抗体（6D1和1H4）与WHcAg之间以及单克隆抗体与HBcAg/HBeAg之间存在交叉反应。感染WHV的土拨鼠和慢性HBV感染患者的肝组织切片都可以被单克隆抗体特异性染色。通过ELISA分析，在WHV和HBV核心蛋白的aa1-8（6D1）和aa125-140（1H4）处定位到表位。WHcAg和HBcAg在aa1-8和aa125-140处分别具有相似的氨基酸序列。