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[拟南芥叶绿体中淀粉降解的新视角]

[New look at starch degradation in Arabidopsis thaliana L. chloroplasts].

作者信息

Samojedny Dorota, Orzechowski Sławomir

机构信息

Katedra Biochemii Wydział Rolnictwa i Biologii, Szkoła Główna Gospodarstwa Wiejskiego, Warszawa.

出版信息

Postepy Biochem. 2007;53(1):74-83.

Abstract

Transitory starch is accumulated during the day and is the main source of energy for the cell metabolism during the night. The observed periodical starch degradation has become a model often used by scientist in their experiments. Starch granule degradation could be divided into 2 periods: initiation of degradation and digestion of amylopectin and amylose into maltooligosaccharide and their derivative. Key meaning is attributed in this process to beta-amylaze, product of its activity beta-maltose is transported to the cytosole and there it subjects farthest conversions. It has been demonstrated that a number of enzymes take part in the starch degradation process. However, the way of regulating their activity is still not fully explained. There is most important elements effecting rate of starch decomposition: day cycle, starch phosphorylation and regulation of enzyme activity. It proceeds through redox potential, pH changes and phosphorylation of protein involved in starch degradation due specific phosphatases. The purpose of the current work is to systematize the knowledge of the Arabidopsis thaliana L. leaf starch degradation. The results of the recent research cast a new light on the starch degradation process as well as on its control.

摘要

暂存淀粉在白天积累,是夜间细胞代谢的主要能量来源。观察到的周期性淀粉降解已成为科学家在实验中经常使用的模型。淀粉颗粒降解可分为两个阶段:降解起始阶段以及支链淀粉和直链淀粉被消化成麦芽寡糖及其衍生物的阶段。在此过程中,关键作用归因于β-淀粉酶,其活性产物β-麦芽糖被转运到细胞质中,并在那里进行进一步转化。已经证明,许多酶参与淀粉降解过程。然而,它们活性的调节方式仍未得到充分解释。影响淀粉分解速率的最重要因素有:昼夜循环、淀粉磷酸化和酶活性调节。这一过程通过氧化还原电位、pH变化以及特定磷酸酶对参与淀粉降解的蛋白质的磷酸化作用来进行。当前这项工作的目的是将拟南芥叶片淀粉降解的知识系统化。最近的研究结果为淀粉降解过程及其控制提供了新的线索。

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