Wittinghofer F, Krengel U, John J, Kabsch W, Pai E F
Max-Planck-Institut für Medizinische Forschung, Abteilung Biophysik, Heidelberg, Germany.
Environ Health Perspect. 1991 Jun;93:11-5. doi: 10.1289/ehp.919311.
The three-dimensional structure of the active guanosine triphosphate (GTP)-analogue-containing complex of the H-ras-encoded p21 has been determined. It was necessary to correct the topology of p21 as published earlier. The structure analysis shows all of the interactions between protein and GTP and how the important cofactor Mg2+ is bound. From the oncogenic mutants of p21 crystallized, a Gly12 to Arg mutation has been analyzed in detail. It shows that the overall structure of the mutant is not perturbed and that the side chain of Arg12 is coming close to the gamma-phosphate for an interaction.
已确定由H-ras编码的p21含活性鸟苷三磷酸(GTP)类似物复合物的三维结构。有必要修正先前发表的p21拓扑结构。结构分析显示了蛋白质与GTP之间的所有相互作用以及重要辅助因子Mg2+的结合方式。从结晶的p21致癌突变体中,对Gly12突变为Arg的突变进行了详细分析。结果表明,突变体的整体结构未受干扰,且Arg12的侧链靠近γ-磷酸基团以进行相互作用。