Mace A T M, Harrow S J, Ganly I, Brown S M
Department of Otolaryngology, Glasgow University, Glasgow, UK.
Acta Otolaryngol. 2007 Aug;127(8):880-7. doi: 10.1080/00016480601075381.
HSV1716 alone and combined with cisplatin was efficacious in destroying head and neck squamous cell carcinoma (HNSCC) cells. Combination treatment with HSV1716 and cisplatin gave additive efficacy. These results indicate that HSV1716 in combination with cisplatin could be of therapeutic value in HNSCC and warrants further investigation.
HSV1716 is a replication competent herpes simplex virus which selectively replicates and lyses actively dividing cells but not normal or terminally differentiated cells. The objective of this study was to determine the efficacy of HSV1716 alone and in combination with cisplatin in HNSCC.
Three HNSCC cell lines were studied; UM-SCC 14C, UM-SCC 22A and UM-SCC 22B. The permissivity of HSV1716 in these cell lines was determined using multicycle growth experiments. In vitro, cytotoxicity of HSV1716 and cisplatin was determined using an MTS proliferation assay. Isobologram analysis was used to determine the interaction between HSV1716 and cisplatin combination treatment.
The three HNSCC cell lines studied were permissive for HSV1716 replication. Cytotoxicity increased in a dose-dependent fashion in all three cell lines. Cisplatin was non-toxic to the virus. Isobologram analysis showed additive cytotoxicity when HSV1716 was combined with cisplatin in all three cell lines.
单纯疱疹病毒1716（HSV1716）及其与顺铂联合使用对破坏头颈部鳞状细胞癌（HNSCC）细胞均有效。HSV1716与顺铂联合治疗具有相加疗效。这些结果表明，HSV1716与顺铂联合应用可能对头颈部鳞状细胞癌具有治疗价值，值得进一步研究。
HSV1716是一种具有复制能力的单纯疱疹病毒，它能选择性地在活跃分裂的细胞中复制并裂解这些细胞，而对正常细胞或终末分化细胞则无此作用。本研究的目的是确定HSV1716单独使用以及与顺铂联合使用对头颈部鳞状细胞癌的疗效。
研究了三种头颈部鳞状细胞癌细胞系；UM-SCC 14C、UM-SCC 22A和UM-SCC 22B。通过多周期生长实验确定HSV1716在这些细胞系中的易感性。在体外，使用MTS增殖试验确定HSV1716和顺铂的细胞毒性。采用等效线图分析确定HSV1716与顺铂联合治疗之间的相互作用。
所研究的三种头颈部鳞状细胞癌细胞系均允许HSV1716复制。在所有三种细胞系中，细胞毒性均呈剂量依赖性增加。顺铂对该病毒无毒。等效线图分析显示，在所有三种细胞系中，HSV1716与顺铂联合使用时均具有相加细胞毒性。
