Zhang R, Best A, Berger R, Cherian S, Lorenzoni S, Macis E, Raiteri R, Cain R
Max Planck Institute for Polymer Research, Ackermannweg 10, D-55128, Mainz, Germany.
Rev Sci Instrum. 2007 Aug;78(8):084103. doi: 10.1063/1.2775433.
We use a multiwell micromechanical cantilever sensor (MCS) device to measure surface stress changes induced by specific adsorption of molecules. A multiplexed assay format facilitates the monitoring of the bending of 16 MCSs in parallel. The 16 MCSs are grouped within four separate wells. Each well can be addressed independently by different analyte liquids. This enables functionalization of MCS separately by flowing different solutions through each well. In addition, each well contains a fixed reference mirror which allows measuring the absolute bending of MCS. In addition, the mirror can be used to follow refractive index changes upon mixing of different solutions. The effect of the flow rate on the MCS bending change was found to be dependent on the absolute bending value of MCS. Experiments and finite element simulations of solution exchange in wells were performed. Both revealed that one solution can be exchanged by another one after 200 microl volume has flown through. Using this device, the adsorption of thiolated DNA molecules and 6-mercapto-1-hexanol on gold surfaces was performed to test the nanomechanical response of MCS.
我们使用多孔微机械悬臂梁传感器（MCS）装置来测量由分子特异性吸附引起的表面应力变化。一种多重检测形式便于并行监测16个MCS的弯曲情况。这16个MCS被分组在四个独立的孔内。每个孔可以由不同的分析物液体独立寻址。这使得通过让不同溶液流经每个孔来分别对MCS进行功能化成为可能。此外，每个孔都包含一个固定的参考镜，它可以用于测量MCS的绝对弯曲度。另外，该镜子可用于跟踪不同溶液混合时的折射率变化。发现流速对MCS弯曲变化的影响取决于MCS的绝对弯曲值。进行了孔内溶液交换的实验和有限元模拟。两者均表明，在200微升体积的溶液流过之后，一种溶液可以被另一种溶液替换。使用该装置，进行了硫醇化DNA分子和6 - 巯基 - 1 - 己醇在金表面的吸附实验，以测试MCS的纳米力学响应。
