生长激素受体表达在人类结直肠癌的肿瘤发生过程中上调。
Growth hormone receptor expression is up-regulated during tumorigenesis of human colorectal cancer.
作者信息
Wu Xiaoyu, Liu Fukun, Yao Xuequan, Li Weisu, Chen Che
机构信息
Department of Gastrointestinal Tumor Surgery, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, China.
出版信息
J Surg Res. 2007 Dec;143(2):294-9. doi: 10.1016/j.jss.2007.03.056. Epub 2007 Aug 30.
BACKGROUND
The aim of the present study was to analyze the expression of growth hormone receptor (GHR) in the colorectal adenoma-carcinoma sequence to determine whether its expression correlates with the various stages of cancer transformation.
METHODS
GHR distribution was assessed by immunohistochemistry and semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) in normal, premalignant, and malignant colorectal lesions.
RESULTS
Most of the normal mucous tissues and hyperplastic polyps showed no or weak immunoreactivity for GHR. In contrast, most of the adenoma and adenocarcinoma samples reacted strongly or moderately with monoclonal GHR antibodies. In RT-PCR, amplified fragments of the expected sizes (247bp) were detected in 90 of 90 samples examined, and the semiquantitative RT-PCR result showed an up-regulation of GHR mRNA expression during the polyp-adenoma-carcinoma sequence, which was consistent with the immunohistochemical results.
CONCLUSIONS
Our results suggest that growth hormone/GHR plays a role in the development of colorectal carcinoma.
背景
本研究旨在分析生长激素受体（GHR）在结直肠腺瘤 - 癌序列中的表达情况，以确定其表达是否与癌症转化的各个阶段相关。
方法
通过免疫组织化学和半定量逆转录聚合酶链反应（RT-PCR）评估正常、癌前和恶性结直肠病变中GHR的分布。
结果
大多数正常黏膜组织和增生性息肉对GHR无免疫反应或免疫反应较弱。相比之下，大多数腺瘤和腺癌样本与单克隆GHR抗体呈强或中度反应。在RT-PCR中，在所检测的90个样本中的90个检测到预期大小（247bp）的扩增片段，半定量RT-PCR结果显示在息肉 - 腺瘤 - 癌序列中GHR mRNA表达上调，这与免疫组织化学结果一致。
结论
我们的结果表明生长激素/GHR在结直肠癌的发生发展中起作用。
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