杆状病毒介导的心肌肌动蛋白过表达是依赖启动子的。
Overexpression of cardiac actin with baculovirus is promoter dependent.
作者信息
Yates Susan P, Otley Marissa D, Dawson John F
机构信息
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ont., Canada N1G 2W1.
出版信息
Arch Biochem Biophys. 2007 Oct 1;466(1):58-65. doi: 10.1016/j.abb.2007.07.018. Epub 2007 Aug 6.
The influence of the promoter and an N-terminal hexahistidine tag on human cardiac actin (ACTC) expression and function was investigated using four baculovirus constructs. It was found that both non-tagged ACTC and hisACTC expression from the p10 promoter was higher than from the polh promoter. Characterization showed that an N-terminal hexahistidine tag has a negative effect on ACTC. Recombinant ACTC inhibits DNase-I and binds myosin S1, indicative of proper folding. Our data support the hypothesis that the actin protein down-regulates the polh promoter.
使用四种杆状病毒构建体研究了启动子和N端六聚组氨酸标签对人心脏肌动蛋白（ACTC）表达和功能的影响。发现来自p10启动子的未标记ACTC和hisACTC的表达均高于来自多角体蛋白（polh）启动子的表达。特性分析表明，N端六聚组氨酸标签对ACTC有负面影响。重组ACTC抑制脱氧核糖核酸酶I（DNase-I）并结合肌球蛋白S1，表明其折叠正确。我们的数据支持肌动蛋白蛋白下调多角体蛋白启动子这一假说。
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