异二聚体限制性内切酶R.BspD6I小亚基的结晶及初步X射线衍射分析
Crystallization and preliminary X-ray diffraction analysis of the small subunit of the heterodimeric restriction endonuclease R.BspD6I.
作者信息
Kachalova Galina S, Yunusova Alfiya K, Artyukh Rimma I, Rogulin Eugeny A, Perevyazova Tatyana A, Zheleznaya Ludmila A, Matvienko Nickolay I, Bartunik Hans D
机构信息
Max-Planck Unit for Structural Molecular Biology, Hamburg 22607, Germany.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt 9):795-7. doi: 10.1107/S174430910704016X. Epub 2007 Aug 31.
Abstract
The heterodimeric restriction endonuclease R.BspD6I is composed of a small subunit with a cleavage site and a large subunit, containing a recognition domain and a cleavage domain, that may function separately as a monomeric nicking endonuclease. Here, the crystallization of the small subunit and diffraction data collection to 1.5 A resolution are reported.
摘要
异二聚体限制性内切酶R.BspD6I由一个具有切割位点的小亚基和一个大亚基组成，大亚基包含一个识别结构域和一个切割结构域，它们可能作为单体切口内切酶单独发挥作用。本文报道了小亚基的结晶及分辨率达到1.5埃的衍射数据收集情况。
相似文献
1
Crystallization and preliminary X-ray diffraction analysis of the small subunit of the heterodimeric restriction endonuclease R.BspD6I.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt 9):795-7. doi: 10.1107/S174430910704016X. Epub 2007 Aug 31.
2
Peculiarities of the interaction of the restriction endonuclease BspD6I with DNA containing its recognition site.
Biochim Biophys Acta. 2016 Sep;1864(9):1072-1082. doi: 10.1016/j.bbapap.2016.05.008. Epub 2016 May 20.
3
Structural analysis of cysteine-free Nt.BspD6 nicking endonuclease and its functional features.
Biochim Biophys Acta Proteins Proteom. 2022 Mar 1;1870(3):140756. doi: 10.1016/j.bbapap.2022.140756. Epub 2022 Jan 10.
4
Structural analysis of the heterodimeric type IIS restriction endonuclease R.BspD6I acting as a complex between a monomeric site-specific nickase and a catalytic subunit.
J Mol Biol. 2008 Dec 12;384(2):489-502. doi: 10.1016/j.jmb.2008.09.033. Epub 2008 Sep 21.
5
Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex.
Biochemistry (Mosc). 2006 Jul;71(7):815-20. doi: 10.1134/s0006297906070157.
6
A study on endonuclease BspD6I and its stimulus-responsive switching by modified oligonucleotides.
PLoS One. 2018 Nov 26;13(11):e0207302. doi: 10.1371/journal.pone.0207302. eCollection 2018.
7
[Investigation of site-specific DNA binding with nicking endonuclease Nt.BspD6I at single molecule level by atomic force microscopy].
Biofizika. 2012 May-Jun;57(3):432-6.
8
Purification, crystallization and preliminary X-ray analysis of the HsdR subunit of the EcoR124I endonuclease from Escherichia coli.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt 7):582-5. doi: 10.1107/S174430910702622X. Epub 2007 Jun 11.
9
Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Mar 1;61(Pt 3):332-4. doi: 10.1107/S174430910500309X.
10
Crystallization and preliminary X-ray diffraction analysis of the HsdR subunit of a putative type I restriction enzyme from Vibrio vulnificus YJ016.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Oct 1;64(Pt 10):926-8. doi: 10.1107/S1744309108027516. Epub 2008 Sep 30.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex.
Biochemistry (Mosc). 2006 Jul;71(7):815-20. doi: 10.1134/s0006297906070157.
3
Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Mar 1;61(Pt 3):332-4. doi: 10.1107/S174430910500309X.
4
Site-specific DNA-nicking mutants of the heterodimeric restriction endonuclease R.BbvCI.
J Mol Biol. 2005 May 6;348(3):631-40. doi: 10.1016/j.jmb.2005.02.034.
5
ARP/wARP and automatic interpretation of protein electron density maps.
Methods Enzymol. 2003;374:229-44. doi: 10.1016/S0076-6879(03)74011-7.
6
Cloning and sequencing of the gene of site-specific nickase N.BspD6I.
Biochemistry (Mosc). 2003 Sep;68(9):984-7. doi: 10.1023/a:1026008528310.
7
Cloning and analysis of the four genes coding for Bpu10I restriction-modification enzymes.
Nucleic Acids Res. 1998 Feb 15;26(4):1084-91. doi: 10.1093/nar/26.4.1084.
8
Solvent content of protein crystals.
J Mol Biol. 1968 Apr 28;33(2):491-7. doi: 10.1016/0022-2836(68)90205-2.
Zotero 插件