Science. 1977 Apr 22;196(4288):436-8. doi: 10.1126/science.196.4288.436.
Pollen of Brassica exhibited strong self-incompatibility. It did not germinate on, adhere to, or extend into the stigmatic tissues of the female parent plant. In contrast, pollen that had been treated with cycloheximide germinated on and penetrated into self-stigmas to the same degree as untreated pollen placed on crossstigmas (compatible). Germ tubes from cycloheximide-treated pollen did not continue growth through stylar tissues and therefore were unable to effect fertilization.
芸薹属花粉表现出强烈的自交不亲和性。它不会在母本植株的柱头组织上发芽、附着或延伸。相比之下,用环己酰亚胺处理过的花粉在自柱头发芽并穿透,其程度与放在异型柱头(亲和)上的未处理花粉相同。环己酰亚胺处理过的花粉的发芽管不能通过花柱组织继续生长,因此无法进行受精。