血浆不对称二甲基精氨酸与冠状动脉疾病的关系

Song Ying, Qu Xiu-fen, Yu Yan-wei, Luan Tian-zhu, Li Jing-jie, Guo Hong, Yu Yang

Second Ward, Department of Craniological Medicine, First Clinical College, Harbin Medical University, Harbin 150001, China.

Zhonghua Yi Xue Za Zhi. 2007 Jun 12;87(22):1527-30.

OBJECTIVE

To investigate whether the plasma asymmetrical dimethylarginine (ADMA) level correlates with the extent and severity of coronary atherosclerosis.

METHODS

110 consecutive patients undergoing coronary angiography were divided into five groups according to the result thereof: control group (n = 22, with normal coronary artery), mild coronary artery disease (CAD) group (n = 21, with stenosis < 50% of the major coronary arteries), single branch CAD group III (n = 22, with stenosis >/= 50% of one major coronary artery); double branch CAD group IV (n = 23, with stenosis >/= 50% of two major coronary arteries); and multi-branch CAD group (n = 22, with significant stenosis >/= 50% of more than two major coronary arteries or companies with stenosis of left major coronary). ELISA was used to detect the plasma ADMA. Nitric acid reductase method and colorimetry were used to measure the levels of plasma nitric oxide (NO) and nitrogen oxide synthase (NOS). The relationship between the plasma ADMA and severity of CAD was analyzed.

RESULTS

The plasma ADMA levels of in last three a groups were 1.52 micromol/L +/- 0.61 micromol/L, 1.67 micromol/L +/- 0.80 micromol/L, and 2.60 micromol/L +/- 0.62 micromol/L all significantly higher than that of the control group (0.79 micromol/L +/- 0.54 micromol/L, P < 0.01). The plasma NO and NOS levels of the multi-branch CAD group were significantly lower than those of the other groups (all P < 0.01), and there were not significant differences in Plasma NO and NOS levels among the other groups. Multivariate stepwise logistic regression analysis showed that the plasma ADMA level was significantly positively correlated with the severity of coronary atherosclerosis (r = 0.684, P = 0.007) and total cholesterol and triglyceride (r = 0.623 and 0.536 respectively), and significantly negatively correlated with the NO and NOS levels (r = -0.709 and -0.701 respectively).

CONCLUSION

Correlated significantly with the severity of coronary atherosclerosis, the plasma ADMA level may become a novel marker of CAD.

目的

探讨血浆不对称二甲基精氨酸（ADMA）水平与冠状动脉粥样硬化的程度及严重性是否相关。

方法

110例连续接受冠状动脉造影的患者根据造影结果分为五组：对照组（n = 22，冠状动脉正常）、轻度冠状动脉疾病（CAD）组（n = 21，主要冠状动脉狭窄<50%）、单支CAD组III（n = 22，一支主要冠状动脉狭窄≥50%）；双支CAD组IV（n = 23，两支主要冠状动脉狭窄≥50%）；以及多支CAD组（n = 22，两支以上主要冠状动脉显著狭窄≥50%或合并左主干冠状动脉狭窄）。采用酶联免疫吸附测定（ELISA）法检测血浆ADMA。采用硝酸还原酶法和比色法测定血浆一氧化氮（NO）和一氧化氮合酶（NOS）水平。分析血浆ADMA与CAD严重性之间的关系。

结果

后三组的血浆ADMA水平分别为1.52微摩尔/升±0.61微摩尔/升、1.67微摩尔/升±0.80微摩尔/升和2.60微摩尔/升±0.62微摩尔/升，均显著高于对照组（0.79微摩尔/升±0.54微摩尔/升，P<0.01）。多支CAD组的血浆NO和NOS水平显著低于其他组（均P<0.01），其他组之间的血浆NO和NOS水平无显著差异。多因素逐步逻辑回归分析显示，血浆ADMA水平与冠状动脉粥样硬化的严重性显著正相关（r = 0.684，P = 0.007），与总胆固醇和甘油三酯也显著正相关（分别为r = 0.623和0.536），与NO和NOS水平显著负相关（分别为r = -0.709和-0.701）。