Grothues D, Tümmler B
Zentrum Biochemie II, Medizinische Hochschule Hannover, Germany.
Mol Microbiol. 1991 Nov;5(11):2763-76. doi: 10.1111/j.1365-2958.1991.tb01985.x.
A general method for the evaluation of macrorestriction fragment patterns is presented and its applicability to the taxonomy of bacteria is demonstrated for 32 Pseudomonas species. Strains were differentiated at the species and subspecies level by genome size and macrorestriction fragment fingerprints of the chromosome that had been separated on pulsed-field gels. The relatedness of bacteria was ascertained from the similarity of AsnI, DraI, SpeI, SspI or XbaI fragment patterns. In general, the dendrograms calculated from the genome fingerprints corresponded with the phylogenetic classification obtained from phenotypic marker or nucleic acid hybridization analysis, but several exceptions were noted. The techniques and algorithms presented herein are generally applicable to the genome analysis of bacteria, lower eukaryotes, and DNA fragments cloned in yeast artificial chromosomes.
本文提出了一种评估宏观限制性片段模式的通用方法，并针对32种假单胞菌属细菌证明了其在细菌分类学中的适用性。通过基因组大小以及在脉冲场凝胶上分离的染色体的宏观限制性片段指纹图谱，可在种和亚种水平区分菌株。根据AsnI、DraI、SpeI、SspI或XbaI片段模式的相似性确定细菌的亲缘关系。总体而言，由基因组指纹图谱计算得出的树状图与从表型标记或核酸杂交分析获得的系统发育分类相对应，但也发现了一些例外情况。本文介绍的技术和算法通常适用于细菌、低等真核生物以及克隆于酵母人工染色体中的DNA片段的基因组分析。
