Science. 1987 Nov 6;238(4828):804-7. doi: 10.1126/science.238.4828.804.
Tissue culture-derived plants of many species have often been observed to possess both genetic and cytogenetic abnormalities. A high frequency of structurally altered chromosomes in maize (Zea mays L.) plants regenerated from tissue culture led to the prediction that newly activated transposable elements could be detected in regenerated plants. Testcrosses of 1200 progeny from 301 regenerated maize plants confirmed that ten regenerated plants from two independent embryo cell lines contained an active Actransposable element. No active Ac elements were present in the explant sources. Recovery of transposable element activity in regenerated plants indicates that some tissue culture-derived genetic variability may be the result of insertion or excision of transposable elements, or both.
许多物种的组织培养衍生植物常常被观察到具有遗传和细胞遗传学异常。在从组织培养再生的玉米（Zea mays L.）植物中，染色体结构改变的高频率导致预测可在再生植物中检测到新激活的转座元件。对来自 301 个再生玉米植株的 1200 个后代的测交证实，来自两个独立的胚细胞系的 10 个再生植株含有活性 Ac 转座元件。在外植体来源中不存在活性 Ac 元件。在再生植株中恢复转座元件活性表明，一些组织培养衍生的遗传变异可能是转座元件的插入或缺失，或两者兼而有之。
