Wang M
Cancer Institute, Zhejiang Medical University, Hangzhou.
Zhonghua Zhong Liu Za Zhi. 1991 Sep;13(5):335-7.
A human colon cancer cell line Hce-8693 was heterotransplanted in nude mice. polyamine biosynthesis inhibitor alpha-difluoromethylornithine (DFMO) showed a marked reproducible inhibition in this model. The size and weight of transplanted tumor in DFMO group were smaller than those of the control group and the average inhibition rate was 72.8% (P less than 0.001). DFMO showed higher tumor inhibitory rate than 5-Fu (35.4%) (P less than 0.001). Furthermore, DFMO demonstrated less severe bone marrow inhibition in the nude mice than 5-Fu (20.0% vs 53.2%, P less than 0.001). There was no synergistic action in these two drugs at the experimental doses. The concentration of putrescine and spermidine in the serum and tumor tissue in the DFMO group were 70% lower than those of the control group (P less than 0.001). These results indicate that the anti-tumor effect of DFMO might be explained by the inhibition of polyamine biosynthesis and this study provides an experimental basis for future clinical application of DFMO.
将人结肠癌细胞系Hce - 8693异种移植到裸鼠体内。多胺生物合成抑制剂α - 二氟甲基鸟氨酸（DFMO）在该模型中显示出显著且可重复的抑制作用。DFMO组移植瘤的大小和重量均小于对照组，平均抑制率为72.8%（P < 0.001）。DFMO的肿瘤抑制率高于5 - 氟尿嘧啶（5 - Fu，35.4%）（P < 0.001）。此外，DFMO对裸鼠骨髓的抑制作用比5 - Fu轻（20.0%对53.2%，P < 0.001）。在实验剂量下，这两种药物没有协同作用。DFMO组血清和肿瘤组织中腐胺和亚精胺的浓度比对照组低70%（P < 0.001）。这些结果表明，DFMO的抗肿瘤作用可能是通过抑制多胺生物合成来解释的，本研究为DFMO未来的临床应用提供了实验依据。
