Smith Denise, Shimamura Takeshi, Barbera Stephanie, Bejcek Bruce E
Department of Biological Sciences, Western Michigan University, Kalamazoo, MI 49008, USA.
Mol Cell Biochem. 2008 Jan;307(1-2):141-7. doi: 10.1007/s11010-007-9593-4. Epub 2007 Sep 9.
NF-kappaB is a family of transcription factors that have been shown to be elevated in a variety of tumor types and in some cases central to their survival and growth. Here we present evidence that U-87 MG and U-118 MG growth is regulated by NF-kappaB and controlled by PDGF. NF-kappaB activity was suppressed by a dominant negative mutant of the human PDGF type beta receptor and PDGF-B chain neutralizing antibodies. Creation of cell lines that had inducible expression of shRNAs directed against either c-Rel or RelA inhibited growth almost 90% indicating that NF-kappaB plays a central role in glioblastoma growth.
核因子-κB是一类转录因子，已证实在多种肿瘤类型中其表达升高，在某些情况下对肿瘤的存活和生长至关重要。在此我们提供证据表明，U-87 MG和U-118 MG细胞的生长受核因子-κB调控，并受血小板衍生生长因子（PDGF）控制。人β型血小板衍生生长因子受体的显性负性突变体和PDGF-B链中和抗体可抑制核因子-κB活性。构建针对c-Rel或RelA的短发夹RNA（shRNA）可诱导表达的细胞系，其生长抑制近90%，表明核因子-κB在胶质母细胞瘤生长中起核心作用。
