大鼠子宫内膜异位症模型的基因表达谱分析。

Gene expression profiling of the rat endometriosis model.

作者信息

Konno Ryo, Fujiwara Hiroyuki, Netsu Sachiho, Odagiri Kohei, Shimane Miyuki, Nomura Hitoshi, Suzuki Mitsuaki

机构信息

Department of Gynecology, Omiya Medical Center Jichi Medical University, Amanuma-cho, Omiya, Saitama, Japan.

出版信息

Am J Reprod Immunol. 2007 Oct;58(4):330-43. doi: 10.1111/j.1600-0897.2007.00507.x.

DOI:10.1111/j.1600-0897.2007.00507.x

PMID:17845203

Abstract

PROBLEM

To investigate the molecular mechanism of endometriosis, gene expression profiling was analyzed in a rat endometriosis model.

METHOD OF STUDY

An endometriosis model was induced by uterine autotransplantation in the peritoneal cavity on a female-SD rat (8 weeks old). As control samples, the normal uterine tissues were used. The gene expression was compared between endometriotic lesions and normal uterine tissues by cDNA microarray analysis, quantitative real time RT-PCR and immunohistochemistry.

RESULTS

The expression of 71 genes was upregulated and that of 45 genes was downregulated in the endometriotic lesions compared to normal uterine tissues. The upregulated genes included genes encoding cytokines, chemokines, growth factors and cell adhesion molecules. The levels of transcripts of osteopontin, Lyn, Vav1, Runx1, and l-selectin in the endometriotic lesions were 130, 10, 10, 12 and 46-fold higher than the respective levels in the eutopic endometrial samples.

CONCLUSION

The results suggest that osteopontin, Lyn, Vav1, Runx1, and l-selectin play important roles in the pathogenesis of endometriosis.

摘要

问题

为了研究子宫内膜异位症的分子机制，对大鼠子宫内膜异位症模型进行了基因表达谱分析。

研究方法

通过将雌性SD大鼠（8周龄）的子宫自体移植到腹腔内诱导建立子宫内膜异位症模型。以正常子宫组织作为对照样本。通过cDNA微阵列分析、定量实时RT-PCR和免疫组织化学比较子宫内膜异位症病灶与正常子宫组织之间的基因表达。

结果

与正常子宫组织相比，子宫内膜异位症病灶中71个基因的表达上调，45个基因的表达下调。上调的基因包括编码细胞因子、趋化因子、生长因子和细胞粘附分子的基因。子宫内膜异位症病灶中骨桥蛋白、Lyn、Vav1、Runx1和l-选择素的转录水平分别比在位子宫内膜样本中的相应水平高130、10、10、12和46倍。