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冻融循环对甲胎蛋白(AFP)、癌胚抗原(CEA)、糖类抗原125(CA125)及糖类抗原19-9(CA19-9)血清检测结果的影响

Effect of freeze-thaw cycles on serum measurements of AFP, CEA, CA125 and CA19-9.

作者信息

Gao Y-C, Yuan Z-B, Yang Y-D, Lu H-K

机构信息

The Immunoassay Laboratory, Department of Nuclear Medicine, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.

出版信息

Scand J Clin Lab Invest. 2007;67(7):741-7. doi: 10.1080/00365510701297480.

Abstract

AFP, CEA, CA125 and CA19-9 are commonly used serum tumour markers (TMs) in clinical practice, although their quantification by immunoassay may be influenced by pre-analytical sample handling. Though the effect of repetitive freeze-thaw cycles is generally recognized, it is not clear in detail. The present study measured (CLIA) these TMs in serum samples freshly separated and after each of five freeze-thaw cycles, in which the samples were frozen at -40 degrees C for 10 months at cycle 4 and 2 h at other cycles. Statistical analysis with the General Linear Model for Repeated Measures revealed significant decreases in the measurements of the four TMs, with the least decrease of 6.8 % for CA125 and the most decrease of 18.2 % for CA19-9 after the last cycle, and an overwhelming single cycle decrease of mean 7.7 % at cycle 4 for AFP, CEA and CA125, of 7.5 % and 9.3 % at cycles 4 and 5 for CA19-9. So it seems that measurements of AFP, CEA and CA125 are more readily affected by long-term frozen storage compared with frequent freezing-thawing, while CA19-9 is relatively unstable under both conditions.

摘要

甲胎蛋白(AFP)、癌胚抗原(CEA)、糖类抗原125(CA125)和糖类抗原19-9(CA19-9)是临床实践中常用的血清肿瘤标志物(TMs),尽管通过免疫测定对它们进行定量可能会受到分析前样本处理的影响。虽然反复冻融循环的影响已得到普遍认可,但具体情况尚不清楚。本研究采用化学发光免疫分析法(CLIA)对新鲜分离的血清样本以及经过五个冻融循环后的样本中的这些肿瘤标志物进行了检测,其中在第4个循环中样本于-40℃冷冻10个月,在其他循环中冷冻2小时。采用重复测量的一般线性模型进行统计分析发现,这四种肿瘤标志物的测量值均显著下降,最后一个循环后,CA125下降最少,为6.8%,CA19-9下降最多,为18.2%;在第4个循环时,AFP、CEA和CA125平均单次循环下降幅度高达7.7%,CA19-9在第4个和第5个循环时分别下降7.5%和9.3%。因此,与频繁冻融相比,AFP、CEA和CA125的测量似乎更容易受到长期冷冻储存的影响,而CA19-9在这两种情况下都相对不稳定。

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