一种适用于分离小肽的新型双（2-乙基己基）磺基琥珀酸钠-聚丙烯酰胺凝胶电泳系统。

A novel sodium bis(2-ethylhexyl) sulfosuccinate-PAGE system suitable for the separation of small peptides.

作者信息

Lu Xuemei, Yao Yao, Zhang Weican

机构信息

State Key Lab of Microbial Technology, Shandong University, Jinan, PR China.

出版信息

Electrophoresis. 2007 Sep;28(18):3174-7. doi: 10.1002/elps.200600779.

DOI:10.1002/elps.200600779

PMID:17854120

Abstract

A novel sodium bis(2-ethylhexyl) sulfosuccinate-PAGE (AOT-PAGE) system which delivers high resolution and sensitivity for small peptides with molecular masses of 0.8-17 kDa is described. Small peptides migrate more slowly and are less prone to leakage than in conventional SDS-PAGE, thus allowing for the in-gel detection with CBB R 250 of 0.5 mug of peptide. The system is also compatible with electroblotting, activity staining in renatured gels, and the peptide analysis by MALDI-MS. AOT-PAGE is simpler, more rapid, and cheaper than the generally adopted Tricine-SDS-PAGE method.

摘要

描述了一种新型的双（2-乙基己基）磺基琥珀酸钠-聚丙烯酰胺凝胶电泳（AOT-PAGE）系统，该系统对分子量为0.8-17 kDa的小肽具有高分辨率和高灵敏度。与传统的SDS-PAGE相比，小肽在该系统中迁移更慢且更不易渗漏，因此可以用考马斯亮蓝R 250对0.5微克肽进行凝胶内检测。该系统还与电转印、复性凝胶中的活性染色以及通过基质辅助激光解吸电离质谱（MALDI-MS）进行的肽分析兼容。AOT-PAGE比普遍采用的三羟甲基氨基甲烷-SDS-PAGE方法更简单、更快速且更便宜。