Scaloni A, Simmaco M, Bossa F
Dipartimento di Scienze Biochimiche A. Rossi Fanelli, Università La Sapienza, Rome, Italy.
Anal Biochem. 1991 Sep 2;197(2):305-10. doi: 10.1016/0003-2697(91)90396-b.
A chiral reagent, 1-fluoro-2,4-dinitro-5-L-alanine, was synthesized for the analysis of enantiomeric mixtures of amino acids after precolumn derivatization. The resulting diastereomers can be separated and quantitated by microbore RP-HPLC. These derivatives are relatively stable under the conditions used for acid hydrolysis of peptide bonds. Thus, this reagent was included in the protocol of a subtractive Edman degradation procedure of peptides to determine the sequence position of amino acid residues with concomitant identification of their chirality at a nanomolar level.
合成了一种手性试剂1-氟-2,4-二硝基-5-L-丙氨酸，用于柱前衍生化后氨基酸对映体混合物的分析。所得非对映异构体可通过微径反相高效液相色谱法进行分离和定量。这些衍生物在肽键酸水解所用的条件下相对稳定。因此，该试剂被纳入肽的减法埃德曼降解程序方案中，以确定氨基酸残基的序列位置，并同时在纳摩尔水平鉴定其手性。
