Imai T, Omoto M
Department of Environmental and Occupational Health, Toho University, School of Medicine.
Arukoru Kenkyuto Yakubutsu Ison. 1991 Dec;26(6):544-68.
The effects of ethanol intake were studied in the three groups of Fischer strain rats. The EtOH-F group began drinking freely at 29 days of age and continued all through mating, pregnancy, delivery, and lactation days. They drank a 10% aqueous solution of ethanol, with net ethyl alcohol 9.7 g/kg b. w. on average. The EtOH-P group drank a 20% aqueous solution of ethanol for the first time on the first day of pregnancy. The solution was then given periodically once a day, oral net ethyl alcohol 3.8 g/kg b. w., through gestation, labor, and lactation. The control group was not exposed to ethanol. Comparative observation were made on both maternal rats and their offspring. 1) Maternal rats: Maternal body weight gain between the first and 20th days of pregnancy was significantly low in the two drinking groups (EtOH-F:p less than 0.01, EtOH-P:p less than 0.01), compared with the control group. Comparing the EtOH-F and the EtOH-P, it was found that body weight gain during the 20 days of pregnancy was significantly depressed in the former group (p less than 0.05). There were no significant differences in placental weight among the three groups. Histological findings disclosed that the brain, liver, and kidney had moderate changes in the EtOH-F, whereas the control showed only slight changes. 2) The average number of fetuses at the 20th day of gestation, neonates per litter, did not significantly different among the three groups. The body weights of fetuses at the 20th day of gestation, of neonates and 10-day-old offspring were recorded. In the case of the EtOH-P group, significantly low values (p less than 0.05, p less than 0.01, p less than 0.01) compared with the respective controls. In the case of the EtOH-F group, the differences were non-significant, p less than 0.01 and p less than 0.01 respectively. 3) Histological study of the brain, lung, liver, kidney, and thymus in offspring revealed that remarkable visceral immaturity and hemorrhage were in the EtOH-P and the EtOH-F groups as compared to the control, and they were more pronounced in the EtOH-F group than in the EtOH-P group. 4) Different ways of ethanol administration brought about different results: The EtOH-P group showed a clearer tendency to have low-body-weight of offspring than those the EtOH-F group.(ABSTRACT TRUNCATED AT 400 WORDS)
在三组Fischer品系大鼠中研究了乙醇摄入的影响。乙醇自由摄入组（EtOH-F组）在29日龄时开始自由饮用乙醇，整个交配、怀孕、分娩和哺乳期都持续饮用。它们饮用10%的乙醇水溶液，平均净乙醇摄入量为9.7克/千克体重。乙醇孕期摄入组（EtOH-P组）在怀孕第一天首次饮用20%的乙醇水溶液。然后在整个妊娠期、分娩期和哺乳期每天定期给予该溶液，口服净乙醇量为3.8克/千克体重。对照组未接触乙醇。对母鼠及其后代进行了对比观察。1）母鼠：与对照组相比，两个饮酒组（EtOH-F组：p<0.01，EtOH-P组：p<0.01）在怀孕第1天至第20天期间母鼠体重增加显著较低。比较EtOH-F组和EtOH-P组发现，前一组在怀孕20天期间体重增加显著降低（p<0.05）。三组胎盘重量无显著差异。组织学检查结果显示，EtOH-F组的脑、肝和肾有中度变化，而对照组仅有轻微变化。2）妊娠第20天的平均胎儿数量，即每窝新生仔鼠数量，三组之间无显著差异。记录了妊娠第20天的胎儿体重、新生仔鼠体重和10日龄后代体重。在EtOH-P组中，与各自对照组相比，数值显著较低（p<0.05，p<0.01，p<0.01）。在EtOH-F组中，差异不显著，分别为p<0.01和p<0.01。3）对后代的脑、肺、肝、肾和胸腺进行组织学研究发现，与对照组相比，EtOH-P组和EtOH-F组存在明显的内脏发育不成熟和出血，且EtOH-F组比EtOH-P组更明显。4）不同的乙醇给药方式产生了不同的结果：EtOH-P组后代体重低的趋势比EtOH-F组更明显。（摘要截选至400字）
