酿酒酵母野生型和突变株中不同的胆色素原脱氨酶形式。与其分离行为的可能关联。

Different porphobilinogen-deaminase forms in wild and mutant strains of Saccharomyces cerevisiae. A possible correlation with its segregants behaviour.

Correa Garcia S R, Rossetti M V, Batlle A M

Centro de Investigaciones sobre Porfirinas y Porfirias-CIPYP (CONICET, F.C.E. y N., UBA), Ciudad Universitaria, Buenos Aires, Argentine.

Int J Biochem. 1991;23(10):1019-23. doi: 10.1016/0020-711x(91)90139-e.

Abstract

Different porphobilinogen-deaminase (PBG-D) enzyme forms were found for D 27 and D 27/C6 (HEM R+) strains of Saccharomyces cerevisiae. 2. PBG-D was partially purified and chromatographed on Sephadex G-100 in either the presence or absence of a protease inhibitor. For D 27 only one active peak was observed while for D 27/C6 strain two active peaks were found. 3. A correlation between this differential behaviour and the presence of HEM R+ gene was looked for employing two segregants of one tetrad from D 27 and D 27/C6 mating.

摘要

发现酿酒酵母的D 27和D 27/C6（HEM R+）菌株存在不同形式的胆色素原脱氨酶（PBG-D）。2. PBG-D在有或无蛋白酶抑制剂的情况下进行部分纯化，并在Sephadex G-100上进行色谱分析。对于D 27仅观察到一个活性峰，而对于D 27/C6菌株则发现两个活性峰。3. 利用来自D 27和D 27/C6杂交的一个四分体的两个分离子，探寻这种差异行为与HEM R+基因存在之间的相关性。