Fuchedzhieva Nadezhda, Karakashev Dimitar, Angelidaki Irini
Bulgarian Academy of Sciences, Institute of Microbiology, Sofia, Bulgaria.
J Hazard Mater. 2008 May 1;153(1-2):123-7. doi: 10.1016/j.jhazmat.2007.08.027. Epub 2007 Aug 15.
Bacillus cereus isolated from municipal wastewater treatment plant was used as a model strain to assess the efficiency of two anionic surfactants, a chemical surfactant and a biosurfactant during fluoranthene biodegradation under anaerobic methanogenic conditions. The surfactants selected for the study were linear alkyl benzene sulphonates (LAS) and rhamnolipid-biosurfactant complex from Pseudomonas sp. PS-17. Biodegradation of fluoranthene was monitored by GC/MS for a period up to 12th day. No change in the fluoranthene concentration was registered after 7th day. The presence of LAS enhanced the cell growth as well as the fluoranthene biodegradation. The rhamnolipid-biosurfactant at both used concentrations inhibited the cell growth and had no effect on the biodegradation rate. It was shown that LAS did not affect the microbial cell permeability and its positive effect on fluoranthene biodegradation was most likely as a result of the increased fluoranthene solubility. The results indicate that LAS can be considered as a promising agent for facilitation of the process of anaerobic polycyclic aromatic hydrocarbons (PAH) biodegradation under methanogenic conditions.
从城市污水处理厂分离出的蜡样芽孢杆菌被用作模型菌株，以评估在厌氧产甲烷条件下，两种阴离子表面活性剂（一种化学表面活性剂和一种生物表面活性剂）在荧蒽生物降解过程中的效率。本研究选用的表面活性剂为直链烷基苯磺酸盐（LAS）和来自假单胞菌属PS - 17的鼠李糖脂 - 生物表面活性剂复合物。通过气相色谱/质谱联用仪（GC/MS）监测荧蒽的生物降解情况，为期12天。第7天后荧蒽浓度无变化。LAS的存在促进了细胞生长以及荧蒽的生物降解。两种使用浓度的鼠李糖脂 - 生物表面活性剂均抑制了细胞生长，且对生物降解速率无影响。结果表明，LAS不影响微生物细胞的通透性，其对荧蒽生物降解的积极作用很可能是由于荧蒽溶解度增加所致。结果表明，LAS可被视为在产甲烷条件下促进厌氧多环芳烃（PAH）生物降解过程的一种有前景的试剂。
