球形芽孢杆菌2362在蚊幼虫循环利用过程中的质粒稳定性

Plasmid stability in Bacillus sphaericus 2362 during recycling in mosquito larvae.

作者信息

Seyler R W, Yousten A A, Burke W F

机构信息

Biology Department, Virginia Polytechnic Institute and State University, Blacksburg 24061-0406.

出版信息

J Invertebr Pathol. 1991 Nov;58(3):362-6. doi: 10.1016/0022-2011(91)90181-o.

DOI:10.1016/0022-2011(91)90181-o

PMID:1787324

Abstract

Bacillus sphaericus 2362 strains transformed with the plasmid pUB110 (4.5 kb) and plasmids derived from it, pLDT103 (7.6 kb) and pLDT117 (9.3 kb), were able to recycle (spore germination, vegetative growth, sporulation) in larvae of Culex quinquefasciatus. During the course of recycling, the pUB110 vector and the recombinant plasmid pLDT103 were stable (100 and 99.2%, respectively). However, the recombinant plasmid pLDT117 exhibited 23% segregational instability. Isolates which lost pLDT117 during recycling retained the one large plasmid native to B. sphaericus 2362.

摘要

用质粒pUB110（4.5 kb）及其衍生质粒pLDT103（7.6 kb）和pLDT117（9.3 kb）转化的球形芽孢杆菌2362菌株能够在致倦库蚊幼虫中循环（孢子萌发、营养生长、孢子形成）。在循环过程中，pUB110载体和重组质粒pLDT103是稳定的（分别为100%和99.2%）。然而，重组质粒pLDT117表现出23%的分离不稳定性。在循环过程中丢失pLDT117的分离株保留了球形芽孢杆菌2362原有的一个大质粒。