Booth Ian R, Edwards Michelle D, Black Susan, Schumann Ulrike, Bartlett Wendy, Rasmussen Tim, Rasmussen Akiko, Miller Samantha
School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, United Kingdom.
Methods Enzymol. 2007;428:47-61. doi: 10.1016/S0076-6879(07)28003-6.
Bacterial mechanosensitive (MS) channels play a significant role in protecting cells against hypoosmotic shock. Bacteria that have been diluted from high osmolarity medium into dilute solution are required to cope with sudden water influx associated with an osmotic imbalance equivalent to 10 to 14 atm. The cell wall is only poorly expansive and the cytoplasmic membrane even less so. Thus, swelling is not an option and the cell must rapidly eject solutes to diminish the osmotic gradient and thereby preserve structural integrity. This chapter describes cellular assays of MS channel function and their interpretation.
细菌机械敏感（MS）通道在保护细胞免受低渗休克方面发挥着重要作用。从高渗培养基稀释到稀溶液中的细菌需要应对与相当于10至14个大气压的渗透失衡相关的突然水流入。细胞壁的扩展性很差，细胞质膜的扩展性更差。因此，肿胀不是一个可行的选择，细胞必须迅速排出溶质以减小渗透梯度，从而保持结构完整性。本章描述了MS通道功能的细胞分析及其解释。
