[多发性骨髓瘤分子细胞遗传学异常的研究]
[Study on molecular cytogenetic abnormalities in multiple myeloma].
作者信息
Liu Shu-Yan, Li Jian-Yong, Chen Li-Juan, Huang Jin-Wen, Pan Jin-Lan, Qiu Hai-Rong, Shen Yun-Feng, Xu Wei, Xue Yong-Quan
机构信息
Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 2007 Apr;28(4):223-6.
OBJECTIVE
To explore the molecular cytogenetic abnormalities in multiple myeloma (MM).
METHODS
Bone marrow plasma cells from 23 previously untreated MM patients were purified by CD138 McAb magnetic cell sorting system, and a panel of probes for interphase fluorescence in situ hybridization were used to detect the 13q14 deletion, p53 deletion and IgH gene translocation in the sorted MM cells.
RESULTS
Among 23 MM patients, 13q14 deletion was observed in 10 (43.5%) cases, with the positive rate of 13q14 deleted cells ranged from 79% to 96%; 14q32 translocation was observed in 11 (47.8%) cases; 13q14 deletion and 14q32 translocation were simultaneously observed in 7 (30.4%) cases; and p53 deletion was observed in none of the 23 cases.
CONCLUSION
The frequency of 13q14 deletion and IgH gene translocation in multiple myeloma are high; and the relationship between 13q14 deletion, IgH gene translocation and prognosis is worth further investigating.
目的
探讨多发性骨髓瘤（MM）的分子细胞遗传学异常。
方法
采用CD138单克隆抗体磁珠分选系统纯化23例初治MM患者的骨髓浆细胞，应用一组间期荧光原位杂交探针检测分选后MM细胞中的13q14缺失、p53缺失及IgH基因易位。
结果
23例MM患者中，10例（43.5%）检测到13q14缺失，13q14缺失细胞阳性率为79%～96%；11例（47.8%）检测到14q32易位；7例（30.4%）同时检测到13q14缺失和14q32易位；23例中均未检测到p53缺失。
结论
多发性骨髓瘤中13q14缺失及IgH基因易位发生率较高；13q14缺失、IgH基因易位与预后的关系值得进一步研究。
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