de Vasconcelos Gurgel Bruno César, Gonçalves Patrícia Furtado, Pimentel Suzana Peres, Ambrosano Gláucia Maria Bovi, Nociti Júnior Francisco Humberto, Sallum Enilson Antonio, Casati Marcio Zaffalon
Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba/Unicamp, São Paulo, Brazil.
Clin Oral Implants Res. 2007 Oct;18(5):649-54. doi: 10.1111/j.1600-0501.2007.01383.x.
The aim of this study was to evaluate histometrically bone healing in surgically created dehiscence-type defects around titanium implants treated with an association of platelet-rich plasma (PRP) and guided bone regeneration (GBR).
Ten male adult mongrel dogs were used, from which the three low premolars (P2, P3, P4) and the first molar were extracted. Three months after teeth extraction, two implant sites were bilaterally drilled, buccal bone dehiscences were created and four titanium implants were placed. Dehiscences were randomly assigned to the following groups: (1) PRP, (2) GBR, (3) PRP+GBR and (4) control. After 3 months, the animals were sacrificed and the implants and adjacent hard tissues were processed for undecalcified sections. Bone-to-implant contact (BIC), bone density within the limits of implant threads (BW), bone density (BD) and new bone area (BA) in a zone lateral to the implant corresponding to bone defects were obtained and measured.
Intergroup analysis (two-way ANOVA -alpha=5%) demonstrated that when PRP was utilized,no differences were observed for all parameters (P>0.05). However, significant differences were observed for BIC and BW toward membrane-treated groups (P<0.05).
Within the limits of this study, it was concluded that PRP does not exert additional effects on bone healing in bone defects created around dental implants and treated by GBR.
本研究旨在通过组织计量学评估富含血小板血浆（PRP）与引导骨再生（GBR）联合应用治疗钛种植体周围手术造成的裂开型骨缺损的骨愈合情况。
选用10只成年雄性杂种犬，拔除其三颗下颌前磨牙（P2、P3、P4）和第一磨牙。拔牙后3个月，双侧钻出两个种植位点，制造颊侧骨裂开，并植入四颗钛种植体。将骨裂开随机分为以下几组：（1）PRP组，（2）GBR组，（3）PRP + GBR组和（4）对照组。3个月后，处死动物，对种植体及相邻硬组织进行处理以制作不脱钙切片。获取并测量种植体与骨的接触面积（BIC）、种植体螺纹范围内的骨密度（BW）、骨密度（BD）以及种植体外侧对应骨缺损区域的新骨面积（BA）。
组间分析（双向方差分析 -α = 5%）表明，使用PRP时，所有参数均未观察到差异（P>0.05）。然而，与使用膜的治疗组相比，BIC和BW观察到显著差异（P<0.05）。
在本研究范围内，得出结论：PRP对牙种植体周围手术造成并经GBR治疗的骨缺损的骨愈合没有额外作用。
