Futakuchi H, Ueda M, Kanda K, Fujino K, Yamaguchi H, Noda S
Department of Obstetrics and Gynecology, Osaka Medical College, Osaka, Japan.
Int J Gynecol Cancer. 2007 Sep-Oct;17(5):1092-8. doi: 10.1111/j.1525-1438.2007.00833.x.
The objective of this study was to evaluate transcriptional expression of survivin and the two splice variants (survivin-2B and survivin-DeltaEx3) in cervical carcinomas. The gene expression levels of survivin and its splice variants in 11 human cervical carcinoma cell lines and 20 malignant and 12 normal cervical tissue samples were analyzed using quantitative reverse transcription-polymerase chain reaction analysis. Gene expression levels of survivin and survivin-DeltaEx3 in cell lines were higher than those in normal cervical tissues (P= 0.0193 and 0.0489). Transcript levels of survivin and survivin-DeltaEx3 in carcinoma tissues were also higher than those in normal controls (P= 0.0016 and 0.0011). Gene expression levels of survivin and survivin-DeltaEx3 in adenocarcinomas were statistically higher than those in squamous cell carcinomas (P= 0.0260 and 0.0487). There was no significant difference in survivin-2B gene expression between malignant and normal cervical samples or different histologic types. The ratios of survivin-2B/survivin and survivin-DeltaEx3/survivin in carcinoma tissues were higher than those in normal controls (P= 0.0288 and 0.0081). Interestingly, the ratio of survivin-2B/survivin was increased in the patients with higher stages and with pelvic lymph node metastasis (P= 0.0205 and 0.0437), respectively. We conclude that survivin and its splice variants might be involved in the pathogenesis and development of cervical carcinomas.
本研究的目的是评估生存素及其两种剪接变体（生存素-2B和生存素-ΔEx3）在宫颈癌中的转录表达。使用定量逆转录-聚合酶链反应分析，对11种人宫颈癌细胞系、20份恶性宫颈组织样本和12份正常宫颈组织样本中生存素及其剪接变体的基因表达水平进行了分析。细胞系中生存素和生存素-ΔEx3的基因表达水平高于正常宫颈组织（P = 0.0193和0.0489）。癌组织中生存素和生存素-ΔEx3的转录水平也高于正常对照（P = 0.0016和0.0011）。腺癌中生存素和生存素-ΔEx3的基因表达水平在统计学上高于鳞状细胞癌（P = 0.0260和0.0487）。恶性和正常宫颈样本或不同组织学类型之间，生存素-2B基因表达无显著差异。癌组织中生存素-2B/生存素和生存素-ΔEx3/生存素的比值高于正常对照（P = 0.0288和0.0081）。有趣的是，在分期较高和有盆腔淋巴结转移的患者中，生存素-2B/生存素的比值分别升高（P = 0.0205和0.0437）。我们得出结论，生存素及其剪接变体可能参与宫颈癌的发病机制和发展。
