Yi Lunzhao, He Jun, Liang Yizeng, Yuan Dalin, Gao Haiyan, Zhou Honghao
Research Center of Modernization of Chinese Medicines, Central South University, Changsha 410083, PR China.
Chem Phys Lipids. 2007 Dec;150(2):204-16. doi: 10.1016/j.chemphyslip.2007.08.002. Epub 2007 Aug 17.
Fatty acids, having intimate relationship with type 2 diabetes mellitus (DM2), are not only the main energy source as nutrients, but also signaling molecules in insulin secretion. In this work, we developed a two-step rapid method to comprehensive profiling of esterified fatty acid (EFA) and non-esterified fatty acid (NEFA) using KOH-CH3OH to methylate EFA followed by H2SO4-CH3OH to methylate NEFA. Its applications to fatty acids profiling of type 2 diabetic patients and health controls were also presented. The t-test results informed that 16 NEFAs and 7 EFAs had distinct differences between type 2 diabetes and health controls. Furthermore, quantitative alterations of fatty acids in plasma of type 2 diabetic patients treated with rosiglitazone were obtained by this method. Our research results indicated that the dynamic changes of NEFAs are various. Some decreased linearly, such as C18:0, C18:3n-6 and C22:6, and some changed nonlinearly, such as C18:3n-3 and C22:4. All results informed that fatty acid profiles could provide comprehensive and accurate information for not only discrimination between DM2 patients and health controls, but also evaluation alterations of fatty acids during therapeutic process.
脂肪酸与2型糖尿病（DM2）关系密切，它不仅是作为营养物质的主要能量来源，也是胰岛素分泌中的信号分子。在本研究中，我们开发了一种两步快速方法，先用KOH-CH3OH使酯化脂肪酸（EFA）甲基化，再用H2SO4-CH3OH使非酯化脂肪酸（NEFA）甲基化，以全面分析EFA和NEFA。本文还介绍了该方法在2型糖尿病患者和健康对照者脂肪酸谱分析中的应用。t检验结果表明，16种NEFA和7种EFA在2型糖尿病患者和健康对照者之间存在显著差异。此外，通过该方法还获得了罗格列酮治疗的2型糖尿病患者血浆中脂肪酸的定量变化。我们的研究结果表明，NEFA的动态变化各不相同。一些呈线性下降，如C18:0、C18:3n-6和C22:6，而一些则呈非线性变化，如C18:3n-3和C22:4。所有结果表明，脂肪酸谱不仅可为区分DM2患者和健康对照者提供全面准确的信息，还可为评估治疗过程中脂肪酸的变化提供依据。
