Shimizu Takeyuki, Osaka Yuko, Banri-Koike Chihiro, Yoshida Maiko, Endo Kanako, Furukawa Koji, Oda Masayuki, Murakami Akikazu, Ogawa Shuhei, Abe Ryo, Azuma Takachika
Division of Structural Immunology, Research Institute for Biological Sciences, Tokyo University of Science, Yamazaki 2669, Noda, Chiba 278-0022, Japan.
Int Immunol. 2007 Oct;19(10):1157-64. doi: 10.1093/intimm/dxm080. Epub 2007 Sep 19.
We examined the immune response of Balb/c mice to antigens prepared by conjugating 2-phenyloxazolone (phOx) to a foreign protein, ovalbumin (OVA), or a self-protein, mouse serum albumin (MSA), in order to study how these chemical modifications would affect immune recognition. We found that anti-OVA antibodies and CD4(+) T cells produced by OVA immunization reacted with OVA as well as with phOx-OVA. Anti-phOx antibodies were produced by phOx-OVA immunization and, interestingly, T cells from these mice reacted only with phOx-OVA but not with the intact OVA. These results suggested that the classical model of hapten-carrier immunization, in which B cells specific to hapten are activated with assistance from T cells specific to a carrier protein, might not be a major route for production of anti-hapten antibodies in hapten-carrier immunization. Furthermore, phOx-MSA immunization induced production of anti-phOx antibodies, which could not be accounted for in terms of the assistance of carrier-specific T cells because of the absence of MSA-specific T cells. Therefore, we proposed a new model in which anti-hapten B cells are assisted by T cells specific to the haptenated carrier.
我们检测了Balb/c小鼠对通过将2-苯基恶唑酮（phOx）与外来蛋白质卵清蛋白（OVA）或自身蛋白质小鼠血清白蛋白（MSA）偶联制备的抗原的免疫反应，以研究这些化学修饰如何影响免疫识别。我们发现，OVA免疫产生的抗OVA抗体和CD4(+) T细胞与OVA以及phOx-OVA反应。phOx-OVA免疫产生了抗phOx抗体，有趣的是，这些小鼠的T细胞仅与phOx-OVA反应，而不与完整的OVA反应。这些结果表明，半抗原-载体免疫的经典模型，即对半抗原有特异性的B细胞在对载体蛋白有特异性的T细胞的辅助下被激活，可能不是半抗原-载体免疫中抗半抗原抗体产生的主要途径。此外，phOx-MSA免疫诱导产生了抗phOx抗体，由于不存在MSA特异性T细胞，因此无法用载体特异性T细胞的辅助来解释。因此，我们提出了一个新模型，其中抗半抗原B细胞由对半抗原化载体有特异性的T细胞辅助。
