[5种毒蕈碱受体亚型在未成熟豚鼠巩膜组织中的表达]
[Expression of 5 muscarinic receptor subtypes in the scleral tissue of immature guinea pigs].
作者信息
Liu Qiong, Wu Jie, Wang Xin-mei, Zeng Jun-wen
机构信息
State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Sep;27(9):1327-30.
OBJECTIVE
To investigate the expression of 5 muscarinic receptor subtypes in the scleral tissue of immature guinea pigs.
METHODS
The scleral tissue was collected from six 2-week-old pigmented guinea pigs to determine mRNA expressions of the muscarinic receptors with RT-PCR. Immunofluorescence was used to observe the protein expressions of the 5 muscarinic receptor subtypes.
RESULTS
The mRNAs of the 5 muscarinic receptor subtypes were all detected in the scleral tissue, and the mRNA expression was the highest for M1 subtype. Green fluorescence of M1 to M5 subtypes surrounding the fibroblast nuclei were found in the scleral matrix using laser confocal microscopy.
CONCLUSION
Five muscarinic receptor subtypes are present in the scleral tissues of immature guinea pigs, indicating the involvement of the muscarinic receptors in eye growth regulation.
目的
研究未成熟豚鼠巩膜组织中5种毒蕈碱受体亚型的表达情况。
方法
从6只2周龄的有色豚鼠采集巩膜组织，采用逆转录聚合酶链反应（RT-PCR）测定毒蕈碱受体的mRNA表达。利用免疫荧光观察5种毒蕈碱受体亚型的蛋白表达。
结果
在巩膜组织中检测到了5种毒蕈碱受体亚型的mRNA，其中M1亚型的mRNA表达最高。使用激光共聚焦显微镜在巩膜基质中发现成纤维细胞核周围存在M1至M5亚型的绿色荧光。
结论
未成熟豚鼠的巩膜组织中存在5种毒蕈碱受体亚型，表明毒蕈碱受体参与眼生长调节。
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