带有侧挂苯并冠醚的1,4,7,10-四氮杂环十二烷（环轮烯）金属配合物的合成及DNA切割特性

Synthesis and DNA-cleavage properties of metal complexes of 1,4,7,10-tetraazacyclododecane (cyclen) functionalized with a pendant benzocrown ether.

作者信息

Tan Xin-Yu, Zhang Ji, Huang Yu, Zhang Yu, Zhou Li-Hong, Jiang Ning, Lin Hong-Hui, Wang Na, Xia Chuan-Qin, Yu Xiao-Qi

机构信息

Department of Chemistry, Key Laboratory of Green Chemistry and Technology, (Ministry of Education), Sichuan University, Chengdu 610064, PR China.

出版信息

Chem Biodivers. 2007 Sep;4(9):2190-7. doi: 10.1002/cbdv.200790176.

DOI:10.1002/cbdv.200790176

PMID:17886837

Abstract

The synthesis and DNA-cleavage properties of a series of novel mononuclear Zn(II), Cu(II), and Co(II) complexes 2 of a crown-ether-functionalized cyclen ligand is described. The Cu complex 2b displayed the highest catalytic activity towards pUC 19 DNA. The effects of reaction time, complex concentration, and pH were investigated, showing that 2b readily and efficiently converts supercoiled (type I ) plasmid DNA to nicked (type II) DNA under physiological conditions (37 degrees, pH 7.4).

摘要

本文描述了一系列冠醚功能化环烯配体的新型单核锌（II）、铜（II）和钴（II）配合物2的合成及其DNA切割特性。铜配合物2b对pUC 19 DNA表现出最高的催化活性。研究了反应时间、配合物浓度和pH值的影响，结果表明，在生理条件（37摄氏度，pH 7.4）下，2b能快速有效地将超螺旋（I型）质粒DNA转化为切口（II型）DNA。