Callahan Damien L, Kolev Spas D, O'Hair Richard A J, Salt David E, Baker Alan J M
School of Chemistry, The University of Melbourne, Parkville, VIC 3010, Australia.
Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3010, Australia.
New Phytol. 2007;176(4):836-848. doi: 10.1111/j.1469-8137.2007.02216.x. Epub 2007 Sep 26.
Experimental evidence suggests that nicotianamine (NA) is involved in the complexation of metal ions in some metal-hyperaccumulating plants. Closely-related nickel (Ni)- and zinc (Zn)-hyperaccumulating species were studied to determine whether a correlation exists between the Ni and Zn concentrations and NA in foliar tissues. A liquid chromatography-mass spectrometry (LC-MS) procedure was developed to quantify the NA and amino acid contents using the derivatizing agent 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. A strong correlation emerged between Ni and NA, but not between Zn and NA. Concentrations of NA and L-histidine (His) also increased in response to higher Ni concentrations in the hydroponic solution supplied to a serpentine population of Thlaspi caerulescens. An inversely proportional correlation was found between the iron (Fe) and Ni concentrations in the leaves. Correlations were also found between Zn and asparagine. The results obtained in this study suggest that NA is involved in hyperaccumulation of Ni but not Zn. The inverse proportionality between the Ni and Fe concentrations in the leaf may suggest that Ni and Fe compete for complexation to NA.
实验证据表明，烟酰胺（NA）在一些金属超积累植物中参与金属离子的络合作用。对亲缘关系密切的镍（Ni）超积累植物和锌（Zn）超积累植物进行了研究，以确定叶片组织中Ni和Zn的浓度与NA之间是否存在相关性。开发了一种液相色谱 - 质谱（LC - MS）方法，使用衍生剂6 - 氨基喹啉 - N - 羟基琥珀酰亚胺基氨基甲酸酯来定量NA和氨基酸含量。Ni与NA之间呈现出强相关性，但Zn与NA之间没有。供应给天蓝遏蓝菜蛇纹石种群的水培溶液中Ni浓度升高时，NA和L - 组氨酸（His）的浓度也会增加。叶片中铁（Fe）和Ni的浓度之间存在反比关系。还发现Zn与天冬酰胺之间存在相关性。本研究获得的结果表明，NA参与Ni的超积累，但不参与Zn的超积累。叶片中Ni和Fe浓度之间的反比关系可能表明Ni和Fe竞争与NA的络合。
