变形链球菌毒力特性表达中双组分系统的系统性失活及表型特征分析
Systemic inactivation and phenotypic characterization of two-component systems in expression of Streptococcus mutans virulence properties.
作者信息
Lévesque C M, Mair R W, Perry J A, Lau P C Y, Li Y-H, Cvitkovitch D G
机构信息
Dental Research Institute, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada.
出版信息
Lett Appl Microbiol. 2007 Oct;45(4):398-404. doi: 10.1111/j.1472-765X.2007.02203.x.
AIM
To assess potential function of each two-component signal transduction system in the expression of Streptococcus mutans virulence properties.
METHODS AND RESULTS
For each two-component system (TCS), the histidine kinase-encoding gene was inactivated by a polymerase chain reaction (PCR)-based deletion strategy and the effects of gene disruption on the cell's ability to form biofilms, become competent, and tolerate acid, osmotic, and oxidative stress conditions were tested. Our results demonstrated that none of the mutations were lethal for S. mutans. The TCS-2 (CiaRH) is involved in biofilm formation and tolerance to environmental stresses, the TCS-3 (ScnRK-like) participates in the survival of cells at acidic pH, and the TCS-9 affects the acid tolerance response and the process of streptococcal competence development.
CONCLUSIONS
Our results confirmed the physiological role of the TCS in S. mutans cellular function, in particular the SncRK-like TCS and TCS-9 as they may represent new regulatory systems than can be involved in S. mutans pathogenesis.
SIGNIFICANCE AND IMPACT OF THE STUDY
Multiple TCS govern important biological parameters of S. mutans enabling its survival and persistence in the biofilm community.
目的
评估变形链球菌毒力特性表达中各双组分信号转导系统的潜在功能。
方法与结果
对于每个双组分系统（TCS），通过基于聚合酶链反应（PCR）的缺失策略使组氨酸激酶编码基因失活，并测试基因破坏对细胞形成生物膜、感受态形成以及耐受酸、渗透和氧化应激条件能力的影响。我们的结果表明，这些突变对变形链球菌均无致死性。TCS-2（CiaRH）参与生物膜形成和对环境应激的耐受性，TCS-3（ScnRK样）参与细胞在酸性pH下的存活，TCS-9影响酸耐受反应和链球菌感受态发育过程。
结论
我们的结果证实了TCS在变形链球菌细胞功能中的生理作用，特别是ScnRK样TCS和TCS-9，因为它们可能代表了参与变形链球菌致病机制的新调控系统。
研究的意义和影响
多个TCS控制变形链球菌的重要生物学参数，使其能够在生物膜群落中存活和持续存在。
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