鸡1号染色体上影响生长和脂肪含量的数量性状基因座的单核苷酸多态性定位
SNP mapping of QTL affecting growth and fatness on chicken GGA1.
作者信息
Rao Yousheng, Shen Xu, Xia Mengna, Luo Chenglong, Nie Qinghua, Zhang Dexiang, Zhang Xiquan
机构信息
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China.
出版信息
Genet Sel Evol. 2007 Sep-Oct;39(5):569-82. doi: 10.1186/1297-9686-39-5-569. Epub 2007 Sep 27.
An F2 chicken population was established from a crossbreeding between a Xinghua line and a White Recessive Rock line. A total of 502 F2 chickens in 17 full-sib families from six hatches was obtained, and phenotypic data of 488 individuals were available for analysis. A total of 46 SNP on GGA1 was initially selected based on the average physical distance using the dbSNP database of NCBI. After the polymorphism levels in all F0 individuals (26 individuals) and part of the F1 individuals (22 individuals) were verified, 30 informative SNP were potentially available to genotype all F2 individuals. The linkage map was constructed using Cri-Map. Interval mapping QTL analyses were carried out. QTL for body weight (BW) of 35 d and 42 d, 49 d and 70 d were identified on GGA1 at 351-353 cM and 360 cM, respectively. QTL for abdominal fat weight was on GGA1 at 205 cM, and for abdominal fat rate at 221 cM. Two novel QTL for fat thickness under skin and fat width were detected at 265 cM and 72 cM, respectively.
一个F2代鸡群由杏花鸡品系与隐性白洛克鸡品系杂交建立。从六个批次的17个全同胞家系中获得了总共502只F2代鸡，其中488只个体的表型数据可用于分析。基于NCBI的dbSNP数据库中的平均物理距离，最初在GGA1上选择了总共46个单核苷酸多态性（SNP）。在对所有F0个体（26只）和部分F1个体（22只）的多态性水平进行验证后，有30个信息性SNP可用于对所有F2个体进行基因分型。使用Cri-Map构建连锁图谱，并进行区间作图QTL分析。在GGA1上分别于351 - 353 cM和360 cM处鉴定出35日龄和42日龄、49日龄和70日龄体重（BW）的QTL。腹部脂肪重量的QTL位于GGA1上的205 cM处，腹部脂肪率的QTL位于221 cM处。分别在265 cM和72 cM处检测到两个新的皮下脂肪厚度和脂肪宽度的QTL。
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