Pradier Amandine, Ettelaie Camille
Biomedical Section, Department of Biological Sciences, University of Hull, Hull, UK.
J Vasc Res. 2008;45(1):19-32. doi: 10.1159/000109074. Epub 2007 Sep 26.
The exposure of tissue factor (TF) at the site of injury or trauma is a rapid process that leads to the initiation of blood coagulation as well as homeostatic processes giving rise to vascular repair.
By exposing human endothelial cells to combinations of exogenous TF and factor VIIa (FVIIa) in serum-free medium, the influence of TF concentrations on cellular proliferation and apoptosis was investigated.
Lower concentrations of TF resulted in increased cellular proliferation as well as upregulation of cyclin D1, downregulation of p21 and p27 and induction of tube formation in vitro. Conversely, incubation with higher concentrations of TF resulted in the activation of caspase-3, expression of p53 and Bax, translocation of p53 into the nucleus and induction of DNA fragmentation. Incubation of the cells with TF/FVIIa led to a lower proliferation rate with additional upregulation in p27.
TF seems to have a bifunctional role in determining the fate of endothelial cells, depending on the concentration and the interactions of this protein. The release of TF in the locality of the injured tissue makes this protein an ideal factor for ascertaining the level of injury and determining the fate of the cells.
组织因子（TF）在损伤或创伤部位的暴露是一个快速过程，它会引发血液凝固以及导致血管修复的稳态过程。
通过在无血清培养基中将人内皮细胞暴露于外源性TF和因子VIIa（FVIIa）的组合中，研究TF浓度对细胞增殖和凋亡的影响。
较低浓度的TF导致细胞增殖增加，以及细胞周期蛋白D1上调、p21和p27下调，并在体外诱导管形成。相反，用较高浓度的TF孵育导致半胱天冬酶-3激活、p53和Bax表达、p53转位至细胞核并诱导DNA片段化。用TF/FVIIa孵育细胞导致增殖率降低，p27进一步上调。
TF似乎在决定内皮细胞命运方面具有双功能作用，这取决于该蛋白的浓度及其相互作用。TF在受损组织局部的释放使该蛋白成为确定损伤程度和决定细胞命运的理想因子。
