Department of Food Science and Technology, Cornell University, Ithaca, NY 14853, USA.
Anal Bioanal Chem. 2008 May;391(2):473-8. doi: 10.1007/s00216-007-1551-1. Epub 2007 Sep 27.
Microbiological contamination of foods continues to be a major concern in public health. Biological toxins are one class of important contaminants that can cause various human diseases. Outbreaks related to contamination by biological toxins or toxin-producing microorganisms have made it extremely important to develop rapid (approximately 20 min), sensitive and cost-effective analytical methods. This paper describes the development of a sensitive bioassay for the detection of cholera toxin (CT) in selected seafood samples, using ganglioside-incorporated liposomes. In this study, the assays were run with food samples spiked with various concentrations of CT. The limit of detection (LOD) increased by a factor of about 10-20 in most food samples, compared with the LOD in the buffer system previously reported. However, the LOD of toxins in food samples (8 × 10-3 × 10(3) fg/mL for CT) was still comparable to, or lower than, that previously reported for other assays. The results from this study demonstrate that the bioassays using ganglioside-liposomes can detect the toxin directly in the field screening of food samples rapidly, simply and reliably, without the need for complex instrumentation.
食品微生物污染仍然是公共卫生关注的主要问题。生物毒素是一类重要的污染物，可导致各种人类疾病。与生物毒素或产毒微生物污染有关的爆发，使得开发快速（约 20 分钟）、灵敏和具有成本效益的分析方法变得极为重要。本文描述了一种使用神经节苷脂包被的脂质体检测选定海鲜样品中霍乱毒素（CT）的灵敏生物测定法的开发。在这项研究中，用含有不同浓度 CT 的食品样品进行了测定。与之前报道的缓冲液系统中的检测限相比，大多数食品样品中的检测限（LOD）提高了约 10-20 倍。然而，食品样品中毒素的检测限（CT 为 8×10-3×10(3)fg/mL）与其他测定方法报道的检测限相当，或更低。本研究结果表明，使用神经节苷脂脂质体的生物测定法可在现场快速、简单、可靠地直接检测食品样品中的毒素，而无需复杂的仪器。
