Ons Ellen, Bleyen Nele, Tuntufye Huruma Nelwike, Vandemaele Fréderic, Goddeeris Bruno Maria
Department of Biosystems, Faculty of Bioscience Engineering, Laboratory of Livestock Physiology, Immunology and Genetics, K.U. Leuven, Heverlee, Belgium.
Avian Pathol. 2007 Oct;36(5):411-4. doi: 10.1080/03079450701589183.
Avian pathogenic Escherichia coli are known to cause significant losses in the poultry industry worldwide. Although prophylactic measures based on vaccination are advisable, until now no full heterologous protection against colibacillosis has been achieved. Since iron is an essential nutrient to these bacteria, the aim of this study was to investigate the prevalence of 12 outer-membrane iron receptor genes in 239 pathogenic strains isolated from clinical cases of colibacillosis in chickens. Five multiplex polymerase chain reactions were developed as a tool for efficient screening. Among the 239 avian E. coli isolates, 100% were positive for fhuE and fepA, 96.2% for fiu, 92.9% for cir, 92.5% for iroN, 87.4% for iutA, 63.2% for fecA, 53.1% for fyuA, 46.9% for fhuA, 45.6% for ireA, 41.8% for chuA and 4.6% for iha.
禽致病性大肠杆菌已知会在全球家禽业造成重大损失。尽管基于疫苗接种的预防措施是可取的，但迄今为止尚未实现对大肠杆菌病的完全异源保护。由于铁是这些细菌的必需营养素，本研究的目的是调查从鸡大肠杆菌病临床病例中分离出的239株致病菌株中12种外膜铁受体基因的流行情况。开发了五种多重聚合酶链反应作为有效筛选的工具。在239株禽大肠杆菌分离株中，fhuE和fepA的阳性率为100%，fiu为96.2%，cir为92.9%，iroN为92.5%，iutA为87.4%，fecA为63.2%，fyuA为53.1%，fhuA为46.9%，ireA为45.6%，chuA为41.8%，iha为4.6%。
