聚乳酸-羟基乙酸共聚物纳米颗粒在栗鼠耳蜗中的分布。
Distribution of PLGA nanoparticles in chinchilla cochleae.
作者信息
Ge Xianxi, Jackson Ronald L, Liu Jianzhong, Harper Elizabeth A, Hoffer Michael E, Wassel Ronald A, Dormer Kenneth J, Kopke Richard D, Balough Ben J
机构信息
Spatial Orientation Center, Department of Otolaryngology, Naval Medical Center San Diego, San Diego, CA 92134-5000, USA.
出版信息
Otolaryngol Head Neck Surg. 2007 Oct;137(4):619-23. doi: 10.1016/j.otohns.2007.04.013.
OBJECTIVES
To study the distribution of polylactic/glycolic acid-encapsulated iron oxide nanoparticles (PLGA-NPs) in chinchilla cochleae after application on the round window membrane (RWM).
STUDY DESIGN AND SETTING
Six chinchillas (12 ears) were equally divided into controls (no treatments) and experimentals (PLGA-NP with or without magnetic exposure). After 40 minutes of PLGA-NP placement on the RWM, perilymph was withdrawn from the scala tympani. The RWM and cochleae were fixed with 2.5% glutaraldehyde and processed for transmission electron microscopy.
RESULTS
Nanoparticles were found in cochleae with or without exposure to magnet forces appearing in the RWM, perilymph, endolymph, and multiple locations in the organ of Corti. Electron energy loss spectroscopy confirmed iron elements in nanoparticles.
CONCLUSION
The nanoparticles were distributed throughout the inner ear after application on the chinchilla RWM, with and without magnetic forces.
SIGNIFICANCE
PLGA-NP applied to the RWM may have potential for sustained therapy to the inner ear.
目的
研究聚乳酸/乙醇酸包裹的氧化铁纳米颗粒（PLGA-NPs）应用于圆窗膜（RWM）后在豚鼠耳蜗中的分布情况。
研究设计与设置
将6只豚鼠（12只耳朵）平均分为对照组（未治疗）和实验组（有或无磁暴露的PLGA-NP）。在将PLGA-NP放置在RWM上40分钟后，从鼓阶抽取外淋巴。将RWM和耳蜗用2.5%戊二醛固定，并进行透射电子显微镜检查。
结果
在有或无磁暴露的耳蜗中均发现纳米颗粒，它们出现在RWM、外淋巴、内淋巴以及柯蒂氏器的多个位置。电子能量损失谱证实了纳米颗粒中的铁元素。
结论
在有或无磁力作用下，纳米颗粒应用于豚鼠RWM后分布于整个内耳。
意义
应用于RWM的PLGA-NP可能具有对内耳进行持续治疗的潜力。
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