妊娠小鼠中sFlt-1过表达对后代成年后血压胎儿编程的性别特异性影响。
Gender-specific effect of overexpression of sFlt-1 in pregnant mice on fetal programming of blood pressure in the offspring later in life.
作者信息
Lu Fangxian, Bytautiene Egle, Tamayo Esther, Gamble Phyllis, Anderson Garland D, Hankins Gary D V, Longo Monica, Saade George R
机构信息
Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, University of Texas Medical Branch at Galveston, Galveston, TX 77555-1062, USA.
出版信息
Am J Obstet Gynecol. 2007 Oct;197(4):418.e1-5. doi: 10.1016/j.ajog.2007.06.064.
OBJECTIVE
The purpose of this study was to determine whether fetal programming of adult blood pressure is altered in a previously characterized mouse model of preeclampsia that was induced by sFlt-1.
STUDY DESIGN
CD-1 mouse mothers at day 8 of gestation were injected with an adenovirus carrying Flt 1-3 (10(9) plaque-forming units) or with an adenovirus carrying mFc as control (10(9) plaque-forming units). The resulting pups were followed until 6 months of age, at which time blood pressure (BP) was recorded continuously for 6 days. The offspring weight was also recorded from weaning until adulthood.
RESULTS
BP was significantly higher in the male offspring that were born to sFlt-1-treated mothers compared with the controls. Male offspring from sFlt-1-treated mothers were significantly smaller from weaning until adulthood. However, there were no significant differences in BP and postweaning weight in female offspring between the 2 groups.
CONCLUSION
Our findings highlight the role of the intrauterine environment in the developmental origin of adult disease.
目的
本研究旨在确定在先前已表征的由可溶性血管内皮生长因子受体1（sFlt-1）诱导的子痫前期小鼠模型中，成年血压的胎儿编程是否发生改变。
研究设计
在妊娠第8天，给CD-1小鼠母亲注射携带Flt 1-3的腺病毒（10⁹ 噬斑形成单位）或携带mFc的腺病毒作为对照（10⁹ 噬斑形成单位）。对所产幼崽进行跟踪直至6月龄，此时连续记录6天血压（BP）。从断奶到成年也记录后代体重。
结果
与对照组相比，经sFlt-1处理的母亲所生雄性后代的血压显著更高。经sFlt-1处理的母亲所生雄性后代从断奶到成年明显更小。然而，两组雌性后代的血压和断奶后体重没有显著差异。
结论
我们的研究结果突出了子宫内环境在成人疾病发育起源中的作用。
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