Fernandes Bruno F, Antecka Emilia, dos Passos Gilberto, Burnier Miguel N
Departamento de Oftalmologia e Patologia da McGill University Health Center & Henry C. Witelson Ocular Pathology Laboratory, Montreal, Canadá.
Arq Bras Oftalmol. 2007 Jul-Aug;70(4):593-8. doi: 10.1590/s0004-27492007000400007.
The purpose of our study was to detect circulating malignant cells (CMCs) in oversea-shipped blood samples of patients with uveal melanoma diagnosed in Brazil. METHODOS: Melan-A and tyrosinase were the two markers used for the detection of CMCs, using reverse transcriptase nested polymerase chain reaction (RT-nested-PCR) in 6 patients with uveal melanoma. The expression of beta-actin and GAPDH were used to assess the quality of the material.
Five patients (83.33%) tested positive for the presence of CMCs. The RT-nested-PCR was positive for melan-A in 4 patients (66.7%) and positive for tyrosinase in 4 (66.7%) of the 6 patients. Three (50%) patients were positive for both markers. One (16.7%) patient was negative for both markers. All negative controls were negative.
The quality of the blood samples shipped overseas, from patients with uveal melanoma, was preserved. The detection of CMCs using RT-nested-PCR was positive in the majority of the patients.
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