Suppr超能文献

栖稻假单胞菌中嘧啶合成的调控

Regulation of pyrimidine formation in Pseudomonas oryzihabitans.

作者信息

West Thomas P

机构信息

Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA.

出版信息

J Basic Microbiol. 2007 Oct;47(5):440-3. doi: 10.1002/jobm.200710333.

Abstract

The regulation of pyrimidine formation in the opportunistic human pathogen Pseudomonas oryzihabitans was investigated at the level of enzyme synthesis and at the level of activity for the pyrimidine biosynthetic pathway enzyme aspartate transcarbamoylase. Although pyrimidine supplementation of succinate-grown P. oryzihabitans cells produced little effect on the de novo pyrimidine biosynthetic pathway enzyme activities, pyrimidine limitation experiments undertaken using an orotidine 5'-monophosphate decarboxylase mutant strain isolated from P. oryzihabitans ATCC 43272 indicated that repression of enzyme synthesis by pyrimidines was occurring. Following pyrimidine limitation of the succinate-grown decarboxylase mutant strain cells, aspartate transcarbamoylase and dihydroorotase activities were found to increase by about 3-fold while dihydroorotate dehydrogenase and orotate phosphoribosyltransferase activities were also observed to increase relative to their activities in the mutant strain cells grown on excess uracil. At the level of enzyme activity, aspartate transcarbamoylase in P. oryzihabitans was strongly inhibited by pyrophosphate, ADP, ATP and GTP in the presence of saturating substrate concentrations.

摘要

在机会性人类病原体栖稻假单胞菌中,对嘧啶生物合成途径中酶天冬氨酸转氨甲酰酶的合成水平和活性水平进行了嘧啶形成调控的研究。虽然向以琥珀酸盐为碳源培养的栖稻假单胞菌细胞补充嘧啶对从头嘧啶生物合成途径的酶活性影响很小,但使用从栖稻假单胞菌ATCC 43272分离得到的乳清苷5'-单磷酸脱羧酶突变株进行的嘧啶限制实验表明,嘧啶正在对酶的合成产生阻遏作用。在以琥珀酸盐为碳源培养的脱羧酶突变株细胞受到嘧啶限制后,发现天冬氨酸转氨甲酰酶和二氢乳清酸酶的活性增加了约3倍,同时相对于在过量尿嘧啶上生长的突变株细胞,二氢乳清酸脱氢酶和乳清酸磷酸核糖基转移酶的活性也有所增加。在酶活性水平上,在底物浓度饱和的情况下,栖稻假单胞菌中的天冬氨酸转氨甲酰酶受到焦磷酸、ADP、ATP和GTP的强烈抑制。

相似文献

1
Regulation of pyrimidine formation in Pseudomonas oryzihabitans.
J Basic Microbiol. 2007 Oct;47(5):440-3. doi: 10.1002/jobm.200710333.
2
Regulation of pyrimidine nucleotide biosynthesis in Pseudomonas synxantha.
Antonie Van Leeuwenhoek. 2007 Oct;92(3):353-8. doi: 10.1007/s10482-007-9164-4. Epub 2007 Jun 20.
3
Effect of carbon source on pyrimidine biosynthesis in Pseudomonas oryzihabitans.
J Basic Microbiol. 2010 Aug;50(4):397-400. doi: 10.1002/jobm.201000022.
4
Control of pyrimidine nucleotide formation in Pseudomonas fulva.
Antonie Van Leeuwenhoek. 2010 Mar;97(3):307-11. doi: 10.1007/s10482-009-9410-z. Epub 2010 Jan 12.
5
Influence of carbon source on pyrimidine synthesis in Pseudomonas mendocina.
J Basic Microbiol. 2003;43(6):534-8. doi: 10.1002/jobm.200310297.
6
Pyrimidine biosynthesis in Pseudomonas veronii and its regulation by pyrimidines.
Microbiol Res. 2012 May 20;167(5):306-10. doi: 10.1016/j.micres.2011.10.002. Epub 2011 Nov 22.
7
Control of the pyrimidine biosynthetic pathway in Pseudomonas pseudoalcaligenes.
Arch Microbiol. 1994;162(1-2):75-9. doi: 10.1007/BF00264376.
8
Regulation of pyrimidine nucleotide formation in Pseudomonas taetrolens ATCC 4683.
Microbiol Res. 2004;159(1):29-33. doi: 10.1016/j.micres.2004.01.007.
9
Regulation of the pyrimidine biosynthetic pathway in Pseudomonas mucidolens.
Antonie Van Leeuwenhoek. 2005 Aug;88(2):181-6. doi: 10.1007/s10482-005-3871-5.
10
Effect of carbon source on pyrimidine formation in Pseudomonas fluorescens ATCC 13525.
Microbiol Res. 2005;160(4):337-42. doi: 10.1016/j.micres.2005.02.006.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验