Abe Fumiyoshi
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
FEBS Lett. 2007 Oct 16;581(25):4993-8. doi: 10.1016/j.febslet.2007.09.039. Epub 2007 Sep 29.
Global transcriptional profiles of Saccharomyces cerevisiae were studied following changes in growth conditions to high hydrostatic pressure and low temperature. These profiles were quantitatively very similar, encompassing 561 co-upregulated genes and 161 co-downregulated genes. In particular, expression of the DAN/TIR cell wall mannoprotein genes, which are generally expressed under hypoxia, were markedly upregulated by high pressure and low temperature, suggesting the overlapping regulatory networks of transcription. In support of the role of mannoproteins in cell wall integrity, cells acquired resistance against treatment with SDS, Zymolyase and lethal levels of high pressure when preincubated under high pressure and low temperature.
研究了酿酒酵母在生长条件转变为高静水压和低温后的全球转录谱。这些转录谱在数量上非常相似，包括561个共同上调基因和161个共同下调基因。特别是，通常在缺氧条件下表达的DAN/TIR细胞壁甘露糖蛋白基因的表达在高压和低温下显著上调，这表明转录调控网络存在重叠。为支持甘露糖蛋白在细胞壁完整性中的作用，当细胞在高压和低温下预孵育时，它们获得了对SDS、溶菌酶处理以及致死水平高压的抗性。
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