Fontana Maria Grazia, Rossi Elisa, Bassotti Gabrio, Aquilano Maria Costanza, Cadei Morris, Grigolato Piergiovanni, Villanacci Vincenzo
First Department of Surgery, University of Brescia, Brescia, Italy.
J Gastroenterol Hepatol. 2007 Nov;22(11):1754-9. doi: 10.1111/j.1440-1746.2006.04530.x.
Gastrointestinal stromal tumors (GIST) constitute a group of primary mesenchymal tumors of the gastrointestinal tract, known for their diversity in clinical behavior and the difficulties in determining malignancy and prognosis. This retrospective study evaluated a series of GIST by means of immunohistochemical techniques, flow cytometry and fluorescence in situ hybridization (FISH).
Nine patients with GIST were analyzed for tumor size, mitotic count and CD117, CD34, MIB-1 with immunohistochemistry. In addition, the GIST were tested with FISH for chromosomes 8 and 17 and DNA index was evaluated by flow cytometry.
The findings confirmed the usefulness of CD117 and CD34 in diagnosing GIST and the prognostic role of MIB-1, but do not support a correlation between aneuploidy in flow cytometry and poor outcome. The FISH results suggest close follow-up for patients with benign GIST with a numerical alteration of chromosome 8. The technique could select patients with tumors at high-risk with aneusomy of chromosome 17.
This study shows the possible application of FISH to the evaluation of patients with GIST, in addition to analysis of morphological features.
胃肠道间质瘤（GIST）是一组胃肠道原发性间充质肿瘤，以其临床行为的多样性以及确定恶性程度和预后的困难而闻名。这项回顾性研究通过免疫组织化学技术、流式细胞术和荧光原位杂交（FISH）对一系列GIST进行了评估。
对9例GIST患者的肿瘤大小、有丝分裂计数以及通过免疫组织化学检测CD117、CD34、MIB-1。此外，用FISH检测GIST的8号和17号染色体，并通过流式细胞术评估DNA指数。
研究结果证实了CD117和CD34在诊断GIST中的作用以及MIB-1的预后价值，但不支持流式细胞术中非整倍体与不良预后之间的相关性。FISH结果表明，对于8号染色体有数值改变的良性GIST患者应密切随访。该技术可以筛选出17号染色体非整倍体的高危肿瘤患者。
本研究表明，除形态学特征分析外，FISH在GIST患者评估中具有潜在应用价值。
Zotero 插件