[体重过度增加的孕妇中血管紧张素转换酶（ACE）基因的插入/缺失多态性（I/D）]

Drews Krzysztof, Seremak-Mrozikiewicz Agnieszka, Nowocień Grzegorz, Bogacz Anna, Kurzawińska Grazyna, Kaluba-Skotarczak Agnieszka

Klinika Perinatologii i Chorób Kobiecych Katedry Perinatologii i Ginekologii Uniwersytetu Medycznego im. K. Marcinkowskiego w Poznaniu.

Ginekol Pol. 2007 Jul;78(7):539-43.

INTRODUCTION

The body mass gain is conditioned by lifestyle, as well as many environmental and genetic factors. Recent studies suggest that renin-angiotensin system (RAS) plays a fundamental role in process of growth and differentiation of adipocytes through the acting of angiotensin II and seems to be a significant factor in excessive weight gain development. The purpose of this study was to determine the frequency and significance of insertion/deletion polymorphism (I/D) of the ACE gene in pregnant women with excessive weight gain.

MATERIALS AND METHODS

The examined group consisted of 212 pregnant women, including 107 women with normal (DeltaBMI< or =5) and 105 women with excessive weight gain (DeltaBMI>5). Genomic DNA was extracted from venous blood. The I/D polymorphism of ACE gene was determined by polymerase chain reaction (PCR).

RESULTS

During the course of the study we did not observe the statistically significant higher frequency of ACE genotypes in any of the two investigated groups of women with normal and excessive weight gain. Nevertheless, an overrepresentation of II genotype frequency in group with excessive weight gain has been observed (33.3 vs 21.5%, p=ns). The same findings were visible as far as the frequency of I allele in group with excessive weight gain was concerned (55.2 vs 45.8%, p=ns). The frequency of observed genotypes was in agreement with Hardy-Weinberg equilibrium.

CONCLUSIONS

Although overrepresentation of II genotype and I allele in the group of pregnant women with excessive weight gain (DeltaBMI>5) has been observed, a close correlation between II genotype and higher risk of overweight could be not indicated (due to the lack of significant difference). The results should be confirmed in a more numerous group of pregnant women. At this stage the results of the study did not suggested the presence of association of I/D polymorphism of ACE gene with weight gain in investigated group of pregnant women.

引言

体重增加受生活方式以及许多环境和遗传因素的影响。最近的研究表明，肾素 - 血管紧张素系统（RAS）通过血管紧张素II的作用在脂肪细胞的生长和分化过程中起重要作用，并且似乎是体重过度增加发展的一个重要因素。本研究的目的是确定体重过度增加的孕妇中ACE基因插入/缺失多态性（I/D）的频率及其意义。

材料与方法

研究对象为212名孕妇，其中107名体重正常（DeltaBMI≤5），105名体重过度增加（DeltaBMI>5）。从静脉血中提取基因组DNA。通过聚合酶链反应（PCR）确定ACE基因的I/D多态性。

结果

在研究过程中，我们未观察到在体重正常和体重过度增加的两组被调查女性中，ACE基因型的频率有统计学上的显著差异。然而，在体重过度增加的组中观察到II基因型频率过高（33.3%对21.5%，p=无显著性差异）。就体重过度增加组中I等位基因的频率而言，也有相同的发现（55.2%对45.8%，p=无显著性差异）。观察到的基因型频率符合哈迪 - 温伯格平衡。