Pontén Eva, Lindström Mona, Kadi Fawzi
Department of Pediatric Orthopaedic Surgery, Karolinska University Hospital, Stockholm, Sweden.
J Neurol Sci. 2008 Mar 15;266(1-2):51-6. doi: 10.1016/j.jns.2007.08.040. Epub 2007 Oct 3.
Spastic cerebral palsy can be divided into diagnostic groups by the relative severity of the arm impairment. This study investigates if hemiplegic, tetraplegic or diplegic cerebral palsy (CP) results in different patterns of myosin heavy chain (MyHC) expression in the flexor carpi ulnaris muscle from 17 young patients with CP. Using enzyme-immunohistochemistry and gel electrophoresis techniques we found a higher percentage of fibers expressing fast MyHC IIx (52%) in tetraplegic CP compared to hemiplegic patients (32%), (p<0.05). Tetraplegic CP also resulted in a lower amount of fibers expressing slow MyHC I (18%) compared to hemiplegic CP (40%), (p<0.005). The proportion of muscle fibers containing fetal MyHC was higher in tetraplegic CP compared to other groups, (p<0.005). Taken together theses results indicate that tetraplegic CP is associated with a shift from slow to fast myosins and that regenerative events are more prominent in tetraplegic CP compared with milder brain damage.
痉挛性脑瘫可根据手臂损伤的相对严重程度分为不同的诊断组。本研究调查了17例年轻脑瘫患者的偏瘫型、四肢瘫型或双瘫型脑瘫是否会导致尺侧腕屈肌中肌球蛋白重链（MyHC）表达模式的差异。使用酶免疫组织化学和凝胶电泳技术，我们发现与偏瘫患者（32%）相比，四肢瘫型脑瘫患者中表达快速肌球蛋白MyHC IIx的纤维百分比更高（52%），（p<0.05）。与偏瘫型脑瘫（40%）相比，四肢瘫型脑瘫中表达慢速肌球蛋白MyHC I的纤维数量也更低（18%），（p<0.005）。与其他组相比，四肢瘫型脑瘫中含有胎儿型MyHC的肌纤维比例更高，（p<0.005）。综合这些结果表明，四肢瘫型脑瘫与从慢速肌球蛋白向快速肌球蛋白的转变有关，并且与较轻的脑损伤相比，四肢瘫型脑瘫中的再生事件更为突出。
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