环孢素A抑制犬单核细胞中白细胞介素-2、白细胞介素-4和γ-干扰素的信使核糖核酸表达，但不抑制肿瘤坏死因子-α的表达。
Cyclosporine A inhibits the mRNA expressions of IL-2, IL-4 and IFN-gamma, but not TNF-alpha, in canine mononuclear cells.
作者信息
Kobayashi Tetsuro, Momoi Yasuyuki, Iwasaki Toshiroh
机构信息
Department of Veterinary Internal Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
出版信息
J Vet Med Sci. 2007 Sep;69(9):887-92. doi: 10.1292/jvms.69.887.
The effects of the calcineurin inhibitors cyclosporine A (CsA) and FK506 on the mRNA expressions of various cytokines were evaluated in dogs to determine whether the effects of CsA and FK506 in dogs were similar to those in humans. The mRNA expression levels of the cytokines IL-2, IL-4, IFN-gamma and TNF-alpha were measured in PHA-stimulated canine PBMC using real-time RT-PCR after incubation with CsA or FK506 for 5 hr. Both reagents inhibited IL-2, IL-4 and IFN-gamma mRNA expressions in a dose-dependent manner. However, CsA hardly inhibited the mRNA expression of TNF-alpha. These findings are important for assessing the indications of CsA treatment in dogs.
在犬类中评估了钙调神经磷酸酶抑制剂环孢素A（CsA）和FK506对各种细胞因子mRNA表达的影响，以确定CsA和FK506在犬类中的作用是否与在人类中的作用相似。在用CsA或FK506孵育5小时后，使用实时RT-PCR在PHA刺激的犬外周血单个核细胞（PBMC）中测量细胞因子IL-2、IL-4、IFN-γ和TNF-α的mRNA表达水平。两种试剂均以剂量依赖性方式抑制IL-2、IL-4和IFN-γ mRNA的表达。然而，CsA几乎不抑制TNF-α的mRNA表达。这些发现对于评估犬类中CsA治疗的适应症很重要。
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