一种基于聚合酶链式反应-变性梯度凝胶电泳的新型方法，用于鉴定浮游生物16S核糖体DNA以诊断溺水。
A novel PCR-DGGE-based method for identifying plankton 16S rDNA for the diagnosis of drowning.
作者信息
He Fanggang, Huang Daixin, Liu Liang, Shu Xiji, Yin Hui, Li Xiaoting
机构信息
Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, No. 13, Hangkong Road, Wuhan 430030, PR China.
出版信息
Forensic Sci Int. 2008 Apr 7;176(2-3):152-6. doi: 10.1016/j.forsciint.2007.08.005. Epub 2007 Oct 4.
The diagnosis of drowning is one of the most difficult issues in forensic practice. We have developed a sensitive and specific PCR and DGGE method for identifying the 16S rDNA of plankton, which exists ubiquitously in all types of water. In order to evaluate the usefulness of this method for diagnosis of drowning, we used this method for detection of plankton 16S rDNA in drowned rabbits and non-drowned rabbits submerged after death, as well as two human drowning cases. Plankton DNA was identified from lung, liver, kidney, blood and brain of the drowned victims, and the DGGE patterns were helpful in indicating the site of drowning. Plankton DNA was also identified from two lung samples obtained from non-drowned rabbits. The results show that the new PCR-DGGE-based method is a potentially useful tool for diagnosing drowning.
溺水的诊断是法医实践中最困难的问题之一。我们开发了一种灵敏且特异的PCR和DGGE方法，用于鉴定浮游生物的16S rDNA，这种浮游生物普遍存在于所有类型的水中。为了评估该方法在溺水诊断中的实用性，我们将此方法用于检测溺水兔和死后浸水的非溺水兔以及两例人类溺水案例中的浮游生物16S rDNA。在溺水受害者的肺、肝、肾、血液和脑中鉴定出了浮游生物DNA，并且DGGE图谱有助于指示溺水地点。在从非溺水兔获得的两份肺样本中也鉴定出了浮游生物DNA。结果表明，基于PCR-DGGE的新方法是诊断溺水的一种潜在有用工具。
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