Bakunts K A, Vladimirtseva E A, Burkrinskaia A G
Vopr Virusol. 1975 Nov-Dec(6):681-5.
Localization of Sendai virus 50S RNA in Ehrlich ascitic carcinoma cells was studied. At 48 hours postinfection virus-specific 50S RNA was found in the nucleus after 30 min exposure to 3H-uridine, and its amount increased after 1-hour exposure to the precursor reaching 18% of the total nucleus virus-specific RNA. After 2-hour or longer exposure to the precursor the amount of 50S RNA in the nuclei decreased considerably and in the cytoplasm in this gradient region a considerable radioactivity appeared. Rapid utilization of 3H-uridine by the cells under these experimental conditions suggests that 50S RNA is synthesized in the nucleus and migrates to the cytoplasm.
研究了仙台病毒50S RNA在艾氏腹水癌细胞中的定位。感染后48小时，在接触3H-尿苷30分钟后，在细胞核中发现病毒特异性50S RNA，在接触前体1小时后其数量增加，达到细胞核中病毒特异性RNA总量的18%。在接触前体2小时或更长时间后，细胞核中50S RNA的数量显著减少，在这个梯度区域的细胞质中出现了相当多的放射性。在这些实验条件下细胞对3H-尿苷的快速利用表明50S RNA在细胞核中合成并迁移到细胞质中。
