在贝酵母、米卡塔酵母和奇异酵母中作为5-氟邻氨基苯甲酸抗性克隆分离得到的色氨酸突变体谱。
The spectrum of Trp- mutants isolated as 5-fluoroanthranilate-resistant clones in Saccharomyces bayanus, S. mikatae and S. paradoxus.
作者信息
Jones Elizabeth W, Berget Peter B, Burnette James M, Anderson Candice, Asafu-Adjei Denise, Avetisian Seda, Barrie Fatmata, Chen Ruby, Chu Bur, Conroy Samantha, Conroy Sean, Dill Allyson, Eimer Will, Garrity Diane, Greenwood Alexander, Hamilton Tamara, Hucko Simon, Jackson Carmen, Livesey Kristen, Monaco Tiffany, Onorato Christina, Otsuka Mai, Pai Satyan, Schaeffer George, Shung Sharon, Spath Samantha, Stahlman Jonathan, Sweeney Blake, Wiltrout Elizabeth, Yurovsky Daniel, Zonneveld Andrea
机构信息
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
出版信息
Yeast. 2008 Jan;25(1):41-6. doi: 10.1002/yea.1552.
5-Fluoroanthranilic acid (FAA)-resistant mutants were selected in homothallic diploids of three Saccharomyces species, taking care to isolate mutants of independent origin. Mutations were assigned to complementation groups by interspecific complementation with S. cerevisiae tester strains. In all three species, trp3, trp4 and trp5 mutants were recovered. trp1 mutants were also recovered if the selection was imposed on a haploid strain. Thus, FAA selection may be more generally applicable than was previously described.
在三种酿酒酵母的同宗二倍体中筛选出对5-氟邻氨基苯甲酸（FAA）具有抗性的突变体，并注意分离出独立起源的突变体。通过与酿酒酵母测试菌株进行种间互补，将突变分配到互补群中。在所有这三个物种中，均获得了trp3、trp4和trp5突变体。如果在单倍体菌株上进行筛选，也能获得trp1突变体。因此，FAA筛选可能比之前描述的更具普遍适用性。
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